面包酵母中谷胱甘肽抽提方法的研究

Methods for Extracting GSH from Saccharomyces cerevisiae

摘要:

对从面包酵母中抽提还原型谷胱甘肽（GSH）的四种方法进行了研究。发现沸水浴抽提、微波辅助提取、超声波破碎和高压均质破碎方法均能有效地抽提胞内GSH。高压均质破碎法在最佳操作条件下GSH抽提量最高为3.975 mg/g，抽提率达到99.87%；沸水浴抽提法GSH抽提量为3.830 mg/g，抽提率为96.23%；超声波破碎法和微波辅助提取GSH抽提量分别为3.823 mg/g和3.593 mg/g，抽提率分别为96.12%和90.28%。高压均质破碎法是一种绿色加工过程，更适合于工业化应用。

Abstract:

Four methods for extracting GSH from Saccharomyces cerevisiae were studied in this paper. It was found that GSH could be efficiently extracted by boiling water extraction, microwave assistant extraction, ultrasonic disruption and high-pressure homogenization. Under the optimized extraction conditions, the GSH concentrations by high-pressure homogenization, boiling water extraction, ultrasonic disruption and microwave assistant extraction were 3.975 mg/g, 3.830 mg/g, 3.823 mg/g and 3.593 mg/g, respectively. And the extraction rates by the above-mentioned four methods were 99.87%, 96.23%, and 96.12% and 90.28%, respectively. The “green” high-pressure homogenization was the most suitable one for industrial production among the examined extraction methods.