鼠李糖脂高产菌株的亚硝基胍诱变选育研究

Screening of High Rhamnolipid-producing Mutants Induced by NTG

摘要:

采用亚硝基胍辅以鼠李糖对铜绿假单胞菌MIG1.46菌株进行诱变，结合蓝色凝胶平板、油平板和发酵培养测定的选育方法，获得了鼠李糖脂产量达21.270 g/L且遗传性质稳定的菌株，产量比原菌株提高361.0%。

Abstract:

A new NTG-induced mutant of Pseudomonas aeruginosa sp. MIG1.46 was screened using blue gel FP, oil FP and fermentation methods. The yield of rhamnolipid produced by the mutant reached 21.270 g/L, which was 361.0% higher than that by the original strain.