富硒处理对绿豆GSH-Px活性及GSH含量的影响

Effect of Selenium Enrichment Processing on GSH-Px Activity and GSH Content of Mung Bean

摘要:

谷胱甘肽过氧化物酶（GSH-Px），最早是在动物体内发现的一种含硒酶，近年来在许多植物中也发现了该酶的存在，并发现适当浓度的外源硒能提高GSH-Px的活性。本文以绿豆为实验材料，用NaSe2O3作为硒源，对绿豆进行富硒处理，研究适当浓度的NaSe2O3对绿豆GSH-Px活性及绿豆中谷胱甘肽（GSH）含量的影响。结果显示：富硒处理可以促进绿豆GSH-Px的合成；无论是浸泡过程还是萌芽阶段，经富硒处理的绿豆其GSH-Px的活性都比对照样高；特别是在富硒处理5 h后，绿豆GSH-Px活性显著增加，比对照提高了59.38%；而绿豆中GSH的含量则是先下降，后升高。

Abstract:

Glutathione Peroxidose (GSH-Px) is a selenium-dependent enzyme which had been found in animal cells at first, and then has been discovered in many plants in recent years. Researchers has found that preferential concentrations exogenous Se could increase the GSH-Px activity of organism. In order to investigate the relationship between Se and the GSH-Px activity of mung bean, the mung bean had been soaked in a NaSe2O3 solution for 5 hours, and then been germinated in the ambient for a given period. The Se content and the GSH-Px activity of the processed mung bean were determined. Results indicate that exogenous Se increases the activity of GSH-Px in mung bean. Regardless of the immersing stage or the germinating stage, the GSH-Px activity of the selenium processed mung bean is higher than the same of the counterpart. After soaking in the selenium solution for 5 hours, the GSH-Px activity of the mung bean is remarkably increase, 59.38% higher than the same of the counterpart.