番石榴叶中黄酮类物质提取及其抗氧化性研究

Extraction of Flavonoid from the Leaves of Guava and its Antioxidition Activity

摘要:

研究了番石榴叶中黄酮类物质的提取工艺,并用正交实验确定了最佳的提取条件。实验结果表明：番石榴叶中总黄酮类物质的最佳提取条件为原材料经过微波处理3 min，再用40%的乙醇回流3 h，此条件下黄酮得率为9.91%。抗氧化实验表明，番石榴叶黄酮提取物能有效抑制猪油的氧化，对羟自由基和超氧自由基有良好的清除作用。

Abstract:

The extraction conditions of the flavonoid from the leaves of guava was studied and optimized through the orthogonal experiment. The results showed that the optimal microwave treatment time and dipping time in 40 % alcohol were 3 min and 3 h, respectively. Under the optimized conditions, the total flavonoids content, which was measured by spectrophotometic method with rutin as the standard sample, reached 9.91 %. The extracts could efficiently decrease the POV value of the lard and scavenge the radicals of O2-•and •OH.