鱼鳞中糖缀合物的提取与体外抗氧化活性研究

Research on the Isolation of a Glycoconjugate from Fish Scale and Its Antioxidant Activity

摘要:

本文探索了利用碱浸提和酶法从淡水鱼鳞中提取糖缀合物的技术，通过考察NaOH浓度、料液比、提取时间对糖缀合物提取效率的影响，确定了其提取的最佳条件为：0.3 mol/L NaOH溶液、料液比为1:5、室温提取18 h。对提取物初步分析后，进行体外抗氧化活性研究，结果表明鱼鳞糖缀合物对DPPH•、O2.-、•OH有较强的清除能力，同时也有较好的还原能力和抗脂质过氧化能力。

Abstract:

The alkaline and enzymatic extractions of a glycoconjugate from fish scale were researched. The best extraction reagent, the concentration of the extraction reagent, the solid- fluid ratio and the extracting time were found to be NaOH solution, 0.3 mol/L , 1:5 and 18 h, respectively. Evaluation of the antioxidatnt activity of the achieved glycoconjugate showed that the glycoconjugate could efficiently scavenge DPPH•, O2.- and •OH . Besides, it had high reducing capacity and strong ability to prevent lipid peroxidation.