α-葡萄糖苷酶高产菌株诱变选育的研究

Screening of a Mutant Strain Producing Alpha-transglucosidase

摘要:

α-葡萄糖苷酶是生产异麦芽低聚糖的关键酶。本文以α-葡萄糖苷酶产生菌黑曲霉M-1为出发菌株，经UV、UV-LiCl复合诱变和60Co诱变选育，成功筛选出了一株遗传稳定性较好的高产菌株C-1，其产酶活力是出发菌株的2.7倍。有望将来应用于异麦芽低聚糖的生产中。

Abstract:

Alpha-transglucosidase plays a critical role in producing isomalto oligosaccharide. In the paper, A stable mutant strain A.nigerC-1 producing alpha-transglucosidase was obtained by 60Co, UV and UV- LiCl mutant experiments. The productivity of the Alpha-transglucosidase of the mutagenesis is 2.7 times higher than that of the wild strain A.niger M-1.The strain would be applied in the production of isomaltooligosaccharide in the future.