华贵栉孔扇贝全脏器中糖胺聚糖的分离提取及其抗肿瘤活性研究

Extraction and Isolation of Glycosaminoglycan from Chlamys Nobilis and Study on its Anti-tumour Effect

摘要:

以湛江地区盛产的华贵栉孔扇贝全脏器为原料，经木瓜蛋白酶和枯草杆菌中性蛋白酶双酶水解、醇沉后得到华贵栉孔扇贝糖胺聚糖粗品，采用MTT法检测粗品体外对人宫颈癌细胞（Hela）活性的影响。实验结果表明：采用双酶水解法粗品得率为1.26%，总糖含量为41.4%，糖胺聚糖(GAG)含量为26.9%。粗品具有对Hela肿瘤细胞的生长显著性抑制作用(p<0.05)。

Abstract:

Glycosaminoglycan (GAG) from the whole viscera of Chlamys nobilis was achieved via enzymatic hydrolysis by papain and neutral proteinase and ethanol precipitation. Its anti-tumor activity was also examined by MTT colormetric assay. Results showed that the yield of crude product reached 1.26%, and the content of total sugar and GAG were 41.4% and 26.9%, respectively. Besides, it showed significant anti-tumor activity towards Hela cell (p<0.05).