Neuroprotective Effects of A Minor Saponin from Centella asiatica (CA-1) Based on PI3K/Akt Signaling Pathway
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Abstract:
The neuroprotective effects of a minor saponin extracted from Centella asiatica (CA-1) was investigated using the 6-hydroxydopamine (6-OHDA)-induced differentiated PC12 cells as the in vitro Parkinson's disease (PD) cell model. The chemical structure was identified by high-resolution secondary mass spectrometry, and the effects of common saponins and CA-1 in Centella asiatica on the cell viability of PC12 cells were examined. Further, the expressions of related genes and proteins in PC12 cells were detected by techniques such as semi-quantitative or real-time quantitative polymerase chain reaction (PCR) and western blot. The results showed that in this model, 100 μmol/L CA-1, asiaticoside (AS) and madecassoside (MA) increased cell viability by 28.63%, 16.69% and 17.54%, respectively. The neuroprotective effect of CA-1 was stronger than those of AS and MA (p<0.05). Compared with the model group, CA-1 increased the survival rate of 6-OHDA-treated PC12 cells in a dose-dependent manner (which differed significantly from the model group at a CA-1 concentration of 25 μmol/L p<0.05), decreased the intracellular reactive oxygen species (ROS) level, and up-regulated the gene expressions of Sod1, Cat and Bcl2. Western blot showed that CA-1 increased the protein expression levels of P85, PDK1 and Akt. In this study, the neuroprotective effect of CA-1 was confirmed, revealing that this effect may be through the PI3K/Akt signaling pathway.
