Anti - Parkinsonism Effect of Laver Combined with Madopar and Its Effect on the Gut Microbiota
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Abstract:
The aim of this study was to investigate the anti-parkinsonism effect of laver combined with madopar and its effect on intestinal flora. Forty-eight C57BL/6J mice were randomly divided into 4 groups : normal control group, Parkinson's model group, madopar treatment group and laver combined madopar treatment group. After 2 weeks of intervention, PD mice were fed a diet supplemented with 5% laver. Behavioral test, immunohistochemical analysis, western blot analysis and inflammatory factor determination were performed to characterize the brain function of PD mice. Illumina platform was used to double-end sequencing the DNA fragments of mice intestinal bacteria to explore the changes of intestinal flora. The results showed that compared with madopar alone, laver combined with madopar could significantly improve the brain function of PD mice, in which the number of neurons and the expression of tyrosine hydroxylase (TH) in substantia nigra were increased by 46.95% and 52.91%, respectively, and the expression of α-synapse nucleoprotein (α-syn) was decreased by 34.56%. The serum IL-1β content was significantly decreased and the inflammation level was improved. The results of 16SrRNA showed that the intestinal flora richness and diversity of mice were increased in the treatment group of laver combined with madopar. The ratio of Firmicutes to Bacteroidota decreased by 71.59%; Muribaculaceae and Akkermansia bacteria related to anti-inflammatory levels were significantly enriched, while Blautia, Helicobacter and other inflammatory bacteria were significantly decreased in relative abundance. Compared with madopar alone, combined treatment with laver has better effects on regulating intestinal flora and reducing inflammation, and has more significant anti-Parkinson effect.
