摘要：为探究化学修饰对三角褐指藻胞内多糖抑制宫颈癌Hela细胞增殖抑制效果的影响。本研究采用超声破壁法结合热水辅助浸提法提取三角褐指藻胞内多糖PTP （Intracellular Polysaccharide from Phaeodactylum tricornutum），对三角褐指藻胞内多糖PTP进行四种化学修饰（硫酸化修饰、磷酸化修饰、乙酰化修饰和羧甲基化修饰），对三角褐指藻胞内多糖PTP、硫酸化多糖S-PTP、磷酸化多糖P-PTP、乙酰化多糖A-PTP和羧甲基化多糖C-PTP进行抑制宫颈癌Hela细胞增殖活性研究。结果显示，得到的硫酸化多糖S-PTP取代度为0.714，S-PTP 较PTP抑制宫颈癌Hela细胞增殖率最大可提高9.21%、磷酸化多糖P-PTP取代度为0.191, P-PTP 较PTP抑制宫颈癌Hela细胞增殖率最大可提高4.01%、乙酰化多糖A-PTP取代度为0.513, A-PTP 较PTP抑制宫颈癌Hela细胞增殖率最大可提高13.53%，羧甲基化多糖C-PTP取代度为0.915，C-PTP 较PTP抑制宫颈癌Hela细胞增殖率最大可提高8.39%。研究结果显示，四种修饰多糖对Hela细胞增殖抑制效果较PTP均有所提升，化学修饰对Hela细胞增殖抑制提升效果最大的为乙酰化修饰，A-PTP 较PTP抑制宫颈癌Hela细胞增殖率最大可提高13.53%, 达到（46.05±0.85）%。本实验通过对三角褐指藻多糖PTP进行化学修饰，证明三角褐指藻多糖在分子结构上引入新的化学基团对抑制宫颈癌细胞增殖有着较大影响，对进一步研究三角褐指藻的生物活性提供了重要参考，为后续的药物研发提供实验基础。

Abstract : In order to explore the effect of chemical modification on the inhibitory effect of intracellular polysaccharides of Phaeodactylum tricornutum on the proliferation of cervical cancer Hela cells. In this study, the intracellular polysaccharide (PTP) of Phaeodactylum tricornutum was extracted by ultrasonic wall-breaking method combined with hot water-assisted extraction method. Four chemical modifications ( sulfation modification, phosphorylation modification, acetylation modification and carboxymethylation modification ) were carried out on the intracellular polysaccharide (PTP) of Phaeodactylum tricornutum. The intracellular polysaccharide (PTP), sulfated polysaccharide S-PTP, phosphorylated polysaccharide P-PTP, acetylated polysaccharide A-PTP and carboxymethylated polysaccharide C-PTP were studied to inhibit the proliferation of cervical cancer Hela cells. The results showed that the degree of substitution of sulfated polysaccharide S-PTP was 0.714, and the proliferation rate of cervical cancer Hela cells was increased by 9.21 % compared with that of PTP. The degree of substitution of phosphorylated polysaccharide P-PTP was 0.191, and the proliferation rate of cervical cancer Hela cells was increased by 4.01 % compared with that of PTP. The degree of substitution of acetylated polysaccharide A-PTP was 0.513, and the proliferation rate of cervical cancer Hela cells was increased by 13.53 % compared with that of PTP. The degree of substitution of carboxymethylated polysaccharide C-PTP was 0.915. Compared with PTP, C-PTP inhibited the proliferation rate of cervical cancer Hela cells by 8.39 %. The results showed that the inhibitory effect of four modified polysaccharides on the proliferation of Hela cells was higher than that of PTP.Acetylation modification had the greatest inhibitory effect on the proliferation of Hela cells. Compared with PTP, A-PTP inhibited the proliferation rate of cervical cancer Hela cells by 13.53 %, reaching 46.05 % ± 0.85 %. In this experiment, the chemical modification of PTP was carried out to prove that the introduction of new chemical groups into the molecular structure of PTP has a great influence on the inhibition of cervical cancer cell proliferation. It provides an important reference for further study on the biological activity of Phaeodactylum tricornutum and provides an experimental basis for subsequent drug development.

广西自然科学基金项目