HS-GC-MS联用测定河南传统西瓜豆酱中的挥发性成分

Analysis of Volatile Components in HeNanTraditional Watermelon Soybean Paste by HS-GC/MS

摘要:

以河南传统发酵的西瓜豆酱为研究对象,采用自动顶空进样-气质联用法测定其挥发性成分,通过单因素试验分别考察了平衡温度、平衡时间、震动频率及样品氯化钠的添加量对挥发性成分的影响。结果表明,平衡温度90℃,平衡时间50min,震动频率6次/秒,不添加氯化钠为测定较优条件。在此条件下,鉴定出自然发酵西瓜豆酱中挥发性成分有10类共82种,其中醇类12种,酯类10种,烯烃类14种,醛类6种,酮类8种,醚类4种,酸类1种,烷烃类10,含N化合物7种和其他10种。相对含量较高的是烯烃类、醛类和醇类,分别为23.848%,22.537%和19.743%,三者之和占挥发性成分总量的66.12%,其次是酯类和酮类,分别占11.945%和6.839%,它们对传统西瓜豆酱的特征风味起着至关重要的作用。

Abstract:

Taking traditional watermelon soybean paste from Henan as the research object, the volatile components were analyzed by HS-GC/MS. The effects of equilibrium temperature, equilibrium time, vibration frequency and the amount of sodium chloride on the volatile compounds in the kinds were evaluated by single factor experiments. The results showed that the optimum conditions we determination are equilibrium temperature at 90 ℃, equilibrium time for 50 minutes, vibration frequency at 6 times per second and no addition of sodium chloride. Under these conditions, 82 kinds of volatile compounds in naturally fermented watermelon soybean paste were identified, including 12 alcohols, 10 esters, 14 olefins, 6 aldehydes, 8 ketones, 4 ethers, 1 acid, 10 alkanes, 7 nitrogen-containing compounds and 10 other compounds.Alkenes, aldehydes and alcohols are relatively high, accounting for 23.848%, 22.537% and 19.743% respectively. The sum of the three accounts for 66.12% of the total volatile components, followed by esters and ketones, accounting for 11.945% and 6.839% respectively, It can be concleded that these compounds play a vital role in the characteristic flavor of traditional watermelon soybean paste.