研究富含玉米黄质色素橄榄油(OZT)对衰老小鼠的影响。对小鼠以0.5mL/10g剂量颈部皮下注射5%的D-半乳糖(D-galactose)溶液42 d后,构建小鼠亚急性衰老模型,将造模成功的小鼠分为模型组、OZT高中低处理组、橄榄油组(OL)、维生素E阳性(VE)对照组,以生理盐水为CK(0)组。连续灌胃28 d后,研究不同剂量OZT对衰老小鼠的体重、脾脏和胸腺指数、血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)活性和丙二醛(MDA)、IL-2、IL-4、IL-10、IFN-γ生成水平等指标的影响。结果显示,OZT均能有效控制衰老小鼠体重,降低血清中的ALT和AST活性,能显著降低SOD、CAT和GSH-Px的活性,促使IL-2、IL-4、IL-10和IFN-γ生成水平,降低MDA含量,OZT高剂量治疗效果显著优于OL,与VE接近。OZT对D-半乳糖致亚急性衰老动物模型有很好的治疗效果,能有效提高小鼠机体抗氧化能力和增强延缓衰老的作用。

To study the effect of olive oil (OZT) rich in zeaxanthin on aging mice. Subacute aging model of mice was established by subcutaneous injected in the neck 5% D-galactose solution at a dose of 0.5 mL/10g of 6 weeks , the model mice were divided into model group, OZT High, middle and low treatment group, olive oil group (OL) , vitamin E positive (VE) control group. After continuous gavage for 28 days, to investigate the effects of different doses of OZT on body weight, major organ index, serum levels of Superoxide dismutase (SOD) , Catalase (CAT) , Glutathione peroxidase (GSH-PX) , alanine aminotransferase (ALT) , aspartate aminotransferase (AST) and malondialdehyde (MDA) , Interleukin-2(IL-2) , Interleukin-4(IL-4) ,Interleukin-10(IL-10)and interferon-gamma (IFN-γ) in aging mice. The results showed that all of the OZT could effectively control the body weight of aging mice, decreased the activities of ALT and AST in serum, decreased the activities of SOD, CAT and GSH-Px, and promote the production of IL-2, IL-4 and IFN-γ, the effects of high-dose OZT was significantly better than that of OL and similar to that of VE. OZT has a good therapeutic effect on the subacute aging model induced by d-galactose. It can effectively improve the anti-oxidation ability of mice and enhance the effect of delaying aging.

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甘肃省科技重大专项（21ZD4NK045）；甘肃省重点研发计划项目（22YF7FK220）