为探究诺尼果多糖对长期高脂膳食引起的代谢紊乱和肥胖的预防作用及其可能机制，本研究分别通过饲以高脂饲料建立高脂膳食小鼠模型和灌胃广谱抗生素建立伪无菌小鼠模型，分析比较诺尼果多糖干预对高脂膳食小鼠体重、粪便总胆汁酸含量(Total bile acid, TBA)和肠道菌群组成的影响。结果表明与高脂膳食组相比，诺尼果多糖组小鼠体重降低11.91%，粪便总胆汁酸含量增加29.58%。但在伪无菌高脂膳食小鼠中，诺尼果多糖的上述作用未能有效发挥，说明肠道菌群在诺尼果多糖抑制高脂膳食小鼠体重增长，促进小鼠粪便总胆汁酸排出中发挥关键作用。Spearman相关性分析结果表明，粪杆菌(Faecalibaculum)、杜氏乳杆菌(Dubosiella)、鼠杆菌(Muribaculaceae)等是在第4周和第12周均发挥促进TBA排出，抑制小鼠体重增长作用的菌属。本研究结果表明，诺尼果多糖通过促进上述有益肠道菌的增殖，发挥促进高脂膳食小鼠TBA排出，抑制高脂膳食小鼠体重增长的作用。

In order to investigate the preventive effect and its potential mechanism of noni fruit polysaccharide (NP) on metabolism abnormalities and obesity induced by long-term intervention with high-fat diet (HFD), an HFD mouse model and a pseudo germ-free mouse model were established by giving high-fat feed and oral administration with broad-spectrum antibiotics, respectively. Body weight, fecal total bile acid (TBA) contents and intestinal microbiota compositions of experimental mice were analyzed and compared in this study. Results showed that body weight of mice in the NP group was decreased by 11.91% and fecal TBA contents were increased by 29.58% compared to mice in the HFD group. However, above beneficial effects of NP were not found in pseudo germ-free HFD mice, indicating that intestinal microbiota was of great importance for NP to inhibit body weight gain and promote fecal TBA excretion of HFD mice. Spearman correlation analysis outcomes suggested that Faecalibaculum, Dubosiella and Muribaculaceae were found to promote TBA excretion and inhibit body weight gain of HFD mice in both week 4 and week 12. Results of this research indicated that NP promoted TBA excretion and inhibited body weight gain of HFD mice via promoting growth of beneficial microbes.

广东省教育厅项目(2021KQNCX035)；广州市科技计划项目(007105356011)