本研究旨在探讨紫菜联合美多芭的抗帕金森（Parkinson’s disease , PD）作用及其对肠道菌群的影响。以48只C57BL/6J小鼠为研究对象，随机分为4组：正常对照组、帕金森模型组、美多芭治疗组和紫菜联合美多芭治疗组。以添加5%紫菜的饲料对PD小鼠干预2周后，进行行为学测试、免疫组化分析、免疫印迹分析、炎症因子测定；利用Illumina平台对小鼠肠道细菌的 DNA片段进行双端测序。结果表明，相较于单独使用美多芭，紫菜联合美多芭治疗可以显著改善PD小鼠的脑功能，其中黑质中神经元的数量、酪氨酸羟化酶（TH）的表达量分别增加了46.95%、52.91%，α-突触核蛋白（α-syn）的表达量减少了34.56%；显著降低了IL-1β的含量，改善其炎症水平。16SrRNA结果表明，紫菜联合美多芭治疗组小鼠肠道菌群的丰富度提升、多样性增加；Firmicutes / Bacteroidota 的比值降低了71.59%；与抗炎水平相关的Muribaculaceae、Akkermansia菌属显著富集，而Blautia、Helicobacter等炎症相关菌属相对丰度明显下降。相较于单独服用美多芭，紫菜联合治疗后，其调节肠道菌群、减轻炎症的效果更佳，抗帕金森作用更显著。

The aim of this study was to investigate the anti-parkinsonism effect of laver combined with madopar and its effect on intestinal flora. Forty-eight C57BL/6J mice were randomly divided into 4 groups : normal control group, Parkinson's model group, madopar treatment group and laver combined madopar treatment group. After 2 weeks of intervention, PD mice were fed a diet supplemented with 5% laver. Behavioral test, immunohistochemical analysis, western blot analysis and inflammatory factor determination were performed to characterize the brain function of PD mice. Illumina platform was used to double-end sequencing the DNA fragments of mice intestinal bacteria to explore the changes of intestinal flora. The results showed that compared with madopar alone, laver combined with madopar could significantly improve the brain function of PD mice, in which the number of neurons and the expression of tyrosine hydroxylase (TH) in substantia nigra were increased by 46.95% and 52.91%, respectively, and the expression of α-synapse nucleoprotein (α-syn) was decreased by 34.56%. The serum IL-1β content was significantly decreased and the inflammation level was improved. The results of 16SrRNA showed that the intestinal flora richness and diversity of mice were increased in the treatment group of laver combined with madopar. The ratio of Firmicutes to Bacteroidota decreased by 71.59%; Muribaculaceae and Akkermansia bacteria related to anti-inflammatory levels were significantly enriched, while Blautia, Helicobacter and other inflammatory bacteria were significantly decreased in relative abundance. Compared with madopar alone, combined treatment with laver has better effects on regulating intestinal flora and reducing inflammation, and has more significant anti-Parkinson effect.

山东省重点研发计划项目(2019GHY112058)