[关键词]
[摘要]
该研究旨在观察非变性I型胶原蛋白(Non-denatured type-I collagen,NDC-I)对免疫低下模型大鼠免疫功能的调节作用。动物实验采用腹腔注射环磷酰胺法建立免疫低下大鼠模型,观察NDC-I对大鼠的胸腺指数和脾脏指数,白细胞计数和分类,血清中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、白介素-2(IL-2)、白介素-4(IL-4)、白介素-10(IL-10)、干扰素-γ(IFN-γ)和肿瘤坏死因子α(TNF-α),脾脏和胸腺的乳酸脱氢酶(LDH)活性和组织形态学变化。结果显示,在给予NDC-I干预30 d后,与模型组比较,NDC-I组胸腺指数上升(p<0.05),白细胞计数显著提高(p<0.01),血清中IgA、IgG、IL-2、IL-4、IL-10、IFN-γ和TNF-α含量为43.32 µg/mL、283.32 µg/mL、1827.17 ng/L、135.97 pg/mL、113.87 ng/L、2302.44 pg/mL、469.91 ng/L,水平显著升高(p<0.01),脾脏中LDH活性显著上升(p<0.01),脾脏和胸腺组织病理学损伤减轻。结果证明,NDC-I对环磷酰胺致免疫低下大鼠的免疫功能具有一定的调节作用,也为胶原蛋白等相关功能产品的综合开发与充分利用提供了参考依据。
[Key word]
[Abstract]
To investigate the regulatoryeffect of non-denatured type-I collagen (NDC-I) on the immune function inimmunocompromised rats, the immunocompromised model was established by intraperitoneal injection of cyclophosphamide in animal experiments. The thymus index,spleen index, white blood cell countand classification, IgA (immunoglobulin A) level, IgG (immunoglobulin G) level, IL-2 (interleukin-2) level, IL-4 (interleukin-4) level, IL-10 (interleukin-10) level, IFN-γ (interferon-gamma) level, TNF-α (tumor necrosis factor-α) level, activity of lactate dehydrogenase (LDH) in spleen and thymus, as well as histomorphological changes of spleen and thymus, were examined. The results showed compared with the model group, a 30-day NDC-I intervention led to significant increases inthe thymus index (p<0.05) and white blood cell count (p<0.01) of the NDC-I treated group, causingsignificant increases (p<0.01) in their levels of serum IgA, IgG, IL-2, IL-4, IL-10, IFN-γ and TNF-α (as 43.32 µg/mL, 283.32 µg/mL, 1827.17 ng/L, 135.97 pg/mL, 113.87 ng/L, 2302.44 pg/mL and 469.91 ng/L, respectively), and the activity of LDH in spleen. In addition, the histopathological damages of the spleen and thymus werereduced. The obtained results revealed that the NDC-I treatment showed a regulatory effect on the immune function of cyclophosphamide-induced immunocompromised rats. This research also provides a reference for the comprehensive development and full utilization of collagen and other related functional products.
[中图分类号]
[基金项目]
广州创尔生物技术股份有限公司科研攻关项目(RD202006)