通过小鼠实验和菌群体外培养实验探究芹菜素对慢传输型便秘小鼠的通便作用及其对肠道菌群的调节作用。将Balb/c小鼠分为空白组、模型组和芹菜素干预组，用洛哌丁胺建立慢传输型便秘模型，给予200 mg/kg•BW芹菜素干预便秘，观测首粒黑便时间、6 h排便粒数、粪便重量、墨汁推进率、粪便含水率等指标，评价芹菜素的通便效果。通过体外分离培养各组小鼠的肠道细菌并进行菌落计数，评价芹菜素对便秘小鼠肠道菌群的调节作用。与模型组相比，芹菜素干预后小鼠首次排黑便时间减少了25%（p<0.05），6 h排便粒数增加了0.86倍（p<0.05）、粪便重量增加了1.21倍（p<0.05）、墨汁推进率增加了1.05倍（p<0.05）、粪便含水率增加了0.82倍（p<0.05）。体外培养实验发现芹菜素抑制有害菌（肠杆菌和肠球菌）的生长（p<0.05），促进益生菌（乳杆菌和双歧杆菌）的生长（p<0.05）。芹菜素对慢传输型便秘小鼠具有通便作用，可能通过调节肠道菌群达到通便效果。

The laxative effect on slow transit constipation and intestinal flora-regulating function of apigenin were investigated through experiments with mice and in vitro microbial culture. Balb/C mice were divided into a blank control group, model group and apigenin intervention group. The slow transit constipation model was established with loperamide. Apigenin（200 mg/kg BW）was used to intervene constipated mice. The laxative effect of apigenin was evaluated through determining the time for the first black stool, number of stool particles within 6 h, stool weight, ink propulsion rate, and water content of stool. The intestinal bacteria of each group of mice were isolated and cultured in vitro, and colony count was measured to evaluate the intestinal flora-regulating function. Compared with the model group, the apigenin intervention group had a shorter time period for the first black stool (reduced by 25%, p<0.05), more stool particles within 6 h (increased by 0.86 times, p<0.05), higher stool weight (increased by 1.21times, p<0.05), higher ink propulsion rate (increased by 1.05 times, p<0.05), and higher stool water content (increased by 0.82 times, p<0.05). In vitro culture experiments showed that apigenin inhibited the growth of harmful bacteria (Enterobacter and Enterococcus) (p<0.05), and promoted the growth of probiotics (Lactobacillus and Bifidobacterium) (p<0.05). Apigenin has a laxative effect on slow transit constipation in mice, which may be achieved through regulating the intestinal flora of constipated mice.

国家自然科学基金面上项目（81773428）；国家自然科学基金青年项目（81703219）；广州市科技计划项目（201904010404）