[关键词]
[摘要]
本实验以秀丽隐杆线虫(Caenorhabditis elegans)为模型,以红鳘鱼胶为原料,探究红鳘鱼胶(HMYJ)、即食花胶(JSYJ)及鱼胶复配物(YJFP)对秀丽隐杆线虫的寿命、运动能力、应激能力和体内抗氧化活性的影响。结果表明,与空白对照组相比,HMYJ、JSYJ及YJFP分别使线虫的寿命延长了9.09%、9.09%、27.03%;JSYJ和YJFP能促进线虫运动;HMYJ、JSYJ及YJFP均能抵抗紫外应激和热应激的损伤。在紫外应激条件下,YJFP、JSYJ、HMYJ使线虫存活率分别提高了33.33%、22.22%、11.11%;而在热应激条件下,YJFP、 JSYJ、HMYJ使线虫存活率都增大了11.11%。此外,HMYJ、JSYJ及YJFP干预后,线虫体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化酶(GSH-Px)的活性显著提高(p<0.05),丙二醛(MDA)的含量显著降低(p<0.05),其中JSYJ样品组降低了43.20%,HMYJ样品组降低了30.80%,YJFP样品组降低了22.80%。综上,HMYJ、JSYJ及YJFP可通过延长线虫寿命,提升其运动能力,提高线虫抗应激损伤的能力及体内抗氧化酶的活性来减缓线虫衰老,该研究也为鱼胶的开发利用提供了理论基础。
[Key word]
[Abstract]
In this experiment, Caenorhabditis elegans was used as a model, and red slate cod croaker isinglass-s was used as raw material. To investigate the effects of HMYJ, JSYJ and their compound (YJFP) on the life spa-n, motor capacity, stress capacity and antioxidant activity of Caenorhabditis elegan. The results showed that HMYJ, JSYJ and YJFP prolong the life span of nematodes by 9.09%, 9.09% and 27.03%, respectively, compared with th-e blank control group. JSYJ and YJFP could promote nematode motility. HMYJ, JSYJ and YJFP could resist th-e damage of ultraviolet stress and heat stresss. Under ultraviolet stress, YJFP, JSYJ and HMYJ increased the survival rate of nematodes by 33.33%, 22.22% and 11.11%, respectively. YJFP, JSYJ and HMYJ increased the survival rate of nematodes by 11.11%. In additionn, after HMYJ, JSYJ and YJFP intervention, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were significantly increased(p<0.05), and the content of malondialdehyde (MDA) was significantly decreased(p<0.05). Among them, JSYJ sample group decreased by 43.20%, HMYJ sample group decreased by 30.80%, YJFP sample decreased by 22.80%. In conclusion, HMYJ, JSYJ and YJFP can prolong the life span of nematodes, improve their locomotion ability, improve the ability of nematodes to resist stress injury and the activity of antioxidant enzymes in vivo to slow down the aging of nematodes. This study also provides a theoretical basis for the development and utilization of Isinglass.
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