探讨桑叶生物碱（mulberry leaf alkaloids, MLA）对酒精性肝损伤小鼠的改善作用。建立酒精性肝损伤小鼠模型，以MLA（40、80、160 mg/kg·day）干预4周，计算肝脏指数，检测血清甘油三酯（TG）、总胆固醇（TC）、谷丙转氨酶（ALT）、谷草转氨酶（AST）和肝脏中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（SOD）、丙二醛（MDA）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）的含量。与模型组相比，MLA中剂量组小鼠的肝脏指数、血清TG、TC、ALT、AST和肝脏MDA、IL-1β、IL-6、TNF-α水平分别降低11.18%、22.78%、19.19%、28.08%、16.16%、25.07%、23.51%、18.61%、11.78%，肝脏SOD、CAT 、GSH-Px活力分别提高27.47%、15.36%、27.83%；MLA高剂量组小鼠的肝脏指数、血清TG、TC、ALT、AST和肝脏MDA、IL-1β、IL-6、TNF-α水平分别降低15.17%、32.65%、23.16%、30.58%、16.41%、31.97%、27.19%、25.41%、29.59%，肝脏SOD、CAT、GSH-Px活力分别提高38.38%、19.09%、31.09%；MLA中、高剂量组肝脏组织结构损伤明显改善。MLA可以改善小鼠酒精性肝损伤，其作用机制可能与改善肝脏氧化应激和抑制炎症反应有关。

The protective effect of Mulberry leaf alkaloids (MLA) on alcoholic liver injury (ALI) in mice was investigated. ALI mouse model was established and three doses of MLA (40, 80, 160 mg/kg·day) were used for intervention. The levels of triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum were detected. Liver catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and liver malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6) were measured. Compared with the model group, the liver index, serum TG, TC, ALT, AST and liver MDA, IL-1β, IL-6 and TNF-α in the MLA middle dose group were decreased by 11.18%, 22.78%, 19.19%, 28.08%, 16.16%, 25.07%, 23.51%, 18.61% and 11.78%, respectively. Liver SOD, CAT and GSH-Px activities in the MLA middle dose group were increased by 27.47%, 15.36% and 27.83%, respectively. The liver index, serum TG, TC, ALT, AST and the levels of MDA, IL-1β, IL-6 and TNF-α in MLA high dose group decreased by 15.17%, 32.65%, 23.16%, 30.58%, 16.41%, 31.97%, 27.19%, 25.41%, 29.59%, respectively. The activities of liver SOD, CAT and GSH-Px were increased by 38.38%, 19.09% and 31.09%, respectively. The damage of liver tissue structure was significantly improved in MLA medium and high dose groups. MLA can improve alcoholic liver injury in mice, and the mechanism may be related to improving liver oxidative stress and inhibiting inflammatory response.

河南省科技攻关项目(202102310511)，国家重点研发计划（2019YFC1708802），河南中医药大学博士科研基金（BSJJ2018-09），河南中医药大学博士科研基金（00104311-2021-1-132）