本文探究了呕吐毒素抑制HK2细胞的增殖与诱导凋亡蛋白表达作用。不同浓度的呕吐毒素作用于HK2细胞后，采用MTT法检测呕吐毒素对细胞增殖的抑制作用，通过荧光显微镜观察细胞形态学的变化，流式细胞术检测细胞凋亡率的变化，采用Western bloting法检测凋亡蛋白Bax、bcl-2、NF-KB、Caspase-2、3、6、8、9的表达水平。研究结果表明，呕吐毒素可显著抑制HK2细胞的增殖，具有明显的量效关系，15、30、40、60和80μM的呕吐毒素对HK2细胞的抑制率分别为61.82%、53.64%、42.43%、38.66%和36.54%；30μM的呕吐毒素处理HK2细胞0、12、24、48、72和96h后,细胞的存活率分别为99.84%、80.51%、60.25%、52.22%、62.50%和71.34%。15μM、40μM和80μM的呕吐毒素处理细胞24h后，细胞的凋亡率分别为2.30%、30.20%和53.50%。Western bloting结果显示呕吐毒素可上调Bax、Caspase-2、3、6、8、9蛋白表达，抑制了bcl-2和NF-KB的蛋白表达。结果表明，呕吐毒素对HK2细胞的增殖有明显的抑制作用，使得细胞发生凋亡，同时对凋亡蛋白的表达具有一定的调节作用。

In this paper, the role of vomiting toxin in inhibiting proliferation of HK2 cells and inducing apoptotic protein expression.After varying concentrations of vomiting toxin acted on HK2 cells, the inhibitory effect of vomiting toxin on cell proliferation was measured by MTT, cell morphology by fluorescence microscopy, apoptosis rate by flow cytometry and the expression level of apoptotic protein Bax, bcl-2, NF-KB, Caspase-2, 3, 6, 8, 9 by Western bloting.The results showed that vomiting toxin significantly inhibited HK2 cell proliferation, with15, 30, 40, 60, and 80μM inhibiting HK2 cells at 61.82%, 53.63%, 32.63%, 38.66% and 36.54%; 30μM vomiting toxin treated with 0, 12, 24, 48, 72 and 96h were 99.84%, 80.51%, 60.25%, 52.22%, 62.50%, and 71.34%, respectively.After 24 h of vomiting toxin treatment at 15μM, 40μM and 80μM, the apoptosis rates were 2.30%, 30.20%, and 53.50%, respectively.The Western bloting results showed that vomiting toxin upregulated Bax, Caspase-2, 3, 6, 8, 9 protein expression and inhibited the protein expression of bcl-2 and NF-KB.The results showed that vomiting toxin has an obvious inhibitory effect on the proliferation of HK2 cells, causing apoptosis and also regulating the expression of apoptotic protein.

国家自然科学基金项目（面上项目，重点项目，重大项目）