[关键词]
[摘要]
探讨芥子酸对高脂饮食仓鼠脂代谢和氧化应激的影响。比色法分别检测仓鼠血清和肝脏中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-c)、低密度脂蛋白(LDL-c)、游离脂肪酸(NEFA),丙二醛(MDA)和总抗氧化力(T-AOC)水平。蛋白印迹法检测肝脏中PPAR-γ、CPT-1、ACC1、FAS、HMGCR、SREBP2和CYP7A1的表达。与高脂饮食组相比,芥子酸干预显著降低肥胖仓鼠体重(23.86%)、附睾脂肪(20.38%)和肾周脂肪(27.91%)指数(p<0.05);改善血脂水平(至TG:183.51 μmol/L、TC:1481.67 μmol/L、HDL-c:507.81 μmol/L、LDL-c:272.50 μmol/L和NEFA:1.16 ng/L);分别降低MDA水平(至血清:7.85 nmol/L,肝脏:5.47 nmol/L)并提升T-AOC水平(至血清:12.63 U/mL,肝脏:113.72 U/mL)。芥子酸干预还能较高脂饮食组仓鼠显著提高PPAR-γ(2.00倍)、CPT-1(2.61倍)、CYP7A1(1.65倍),并显著抑制FAS(34.38%)、ACC1(52.73%)、SREBP2(53.49%)和HMGCR(32.68%)的表达水平(p<0.05)。本研究提示,芥子酸能够显著改善高脂膳食诱导的仓鼠脂代谢紊乱,这与调控脂代谢相关蛋白的表达和减轻氧化应激水平有关。
[Key word]
[Abstract]
To investigate the effect of sinapic acid on lipid metabolism and oxidative stress in a high-fat diet (HFD) fed Syrian hamsters, the serum and hepatic levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-c), low densitylipoprotein (LDL-c), nonesterified free fatty acids (NEFA) and malondialdehyde (MDA) and total antioxidant capacity (T-AOC) were determined by colorimetric method. The hepatic levels of lipid metabolism related proteins, which including peroxisome proliferator-activated receptor gamma (PPAR-γ) carnitine palmitoyltransferase-1 (CPT-1), acetyl-CoA carboxylase1 (ACC1), fatty acid synthase (FAS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP2) and cytochrome P450 family 7 subfamily A member 1 (CYP7A1) were determined using a Western Blotassay. Administrated with sinapic acid can significantly reduce the weight of HFD hamsters (23.86%), epididymal fat (20.38%) and perirenal fat (27.91%) (p<0.05) compared with HFD fed hamsters. Sinapic acid treatment also attenuated the serum lipid levels (TG: 183.51 μmol/L, TC: 1481.67 μmol/L, HDL-c: 507.81 μmol/L, LDL-c: 272.50 μmol/L and NEFA: 1.16 ng/L). The MDA levels were reduced to 7.85 nmol/L and 5.47 nmol/L, and T-AOC levels were increased to 12.63 U/mL and 113.72 U/mL in serum and liver of the model hamsters, respectively, by the intervention of sinapic acid. In addition, sinapic acid can promote the protein expression of PPAR-γ (2.00 times), CPT-1 (2.61 times), CYP7A1 (1.65 times) and inhibit the protein expression of FAS (34.38%), ACC1 (52.73%), SREBP2 (53.49%) and HMGCR (32.68%) compared with HFD hamsters. Our results suggested that sinapic acid can significantly improve lipid metabolism disorders induced by HFD in hamsters, which is related to the regulation of lipid metabolism-related proteins and the reduction of oxidative stress levels.
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[基金项目]
国家自然科学基金项目(81560530;81760589;81960590);广西自然科学基金项目(2016GXNSFBA380172;2020GXNSFAA159160);人社部“高层次留学人才回国资助计划”(人社厅函[2019]160号);2018年广西高等学校千名中青年骨干教师培育计划资助项目(桂教人[2018]18号);桂林医学院引进人才科研启动基金项目(04010150001)