为了解市售水产品中副溶血性弧菌毒力基因携带情况及药敏性情况，本文采用《GB 4789.7-2013 食品安全国家标准 食品微生物学检验 副溶血性弧菌检验》分离培养方法，利用VITEK 2 compact全自动细菌鉴定系统及荧光PCR从市售水产品中分离鉴定出60株副溶血性弧菌分离株，通过常规PCR对60株分离株中四种毒力基因的分布进行筛查，并用VITEK 2 compact全自动细菌鉴定系统对副溶血性弧菌分离株进行药敏性试验。结果显示，市售水产样品的60株副溶血性弧菌分离株中tlh基因携带率为100.00%、toxR/S基因携带率为100.00%、tdh基因携带率为95.00%，trh基因携带率为65.00%，多重毒力基因携带率高，其中四重毒力基因携带率为61.67%；分析60株分离株对19种抗菌药物的药敏情况发现对氨苄西林有极高的耐药性，耐药率为96.67%，多重耐药现象不突出，仅有4株分离株对2种药物耐药。结果表明，市售水产样品中副溶血性弧菌分离株毒力基因携带率较高，且多数携带多重毒力基因；分离株耐药情况不突出，但对抗菌类药物氨苄西林的耐药率较高，需警惕水产食品中副溶血性弧菌的潜在威胁。
In order to investigate the virulence gene carrying status and drug sensitivity of Vibrio parahaemolyticus in aquatic products, 60 strains of Vibrio parahaemolyticus were isolated and identified from aquatic products by the isolated cultivation method from GB 4789.7-2013, VITEK 2 compact automatic bacteria identification system and real-time PCR. The distribution of 4 virulence genes of Vibrio parahaemolyticus isolates was detected by conventional PCR. Meanwhile, drug susceptibility of the isolates was tested by using VITEK 2 compact automatic bacteria identification system. Results showed that in 60 strains of Vibrio parahaemolyticus isolated from commercial aquatic samples, the gene carrying rates of tlh, toxR/S, tdh, trh were 100.00%, 100.00%, 95.00%, and 65.00%, respectively, and quad-virulence genes carrying rate was 61.67%. Results of drug susceptibility test using 19 types of antibacterial agents indicated that all of the 60 isolates were highly resistant to ampicillin, and the resistance rate was 96.67%. The phenomenon of multiple drug resistance was not prominent, for just only 4 isolates were resistant to 2 drugs. The results indicated that the virulence gene carrying rate of Vibrio parahaemolyticus from the commercial aquatic samples was high, and most of the isolates carried multiple virulence genes. The drug resistance of Vibrio parahaemolyticus from the commercial aquatic samples was not outstanding, but the resistance rate to ampicillin was high, so we should be alert to the potential threat of Vibrio parahaemolyticus in aquatic food.