本论文采用静电纺丝技术制备了醋酸纤维素/聚乙烯吡咯烷酮/ε-聚赖氨酸（cellulose acetate/polyvinylpyrrolidone/ε-polylysine，CA/PVP/ε-PL）抗菌纳米纤维膜，并对纤维膜进行了结构表征和性能研究。结果表明：溶液黏度和电导率对纤维形貌有较大影响，当CA、PVP与ε-PL的质量分数分别为70、35和20 g/L时，纤维膜的直径均匀分布在150~250 nm之间；傅里叶红外光谱、X射线衍射和差示扫描量热仪的结果显示，ε-PL被成功包埋在纤维膜中，且各组分之间具有强烈的相互作用；热重分析结果表明CA/PVP/ε-PL纳米纤维膜的热降解温度升至441.30 ℃，具有良好的热稳定性；抑菌实验结果显示CA/PVP/ε-PL纳米纤维膜的抗菌性优于流延膜，其对大肠杆菌和金黄色葡萄球菌的抑菌圈直径/膜质量比为0.53~0.67 cm/mg，且能延长生鲜肉的贮藏时间3 d以上。综上所述，CA/PVP/ε-PL纳米纤维膜具有良好的抗菌活性，对生鲜肉具有保鲜作用，可用于食品的抗菌保鲜。本研究结果为负载ε-PL纳米抗菌膜的实际应用提供理论依据。

In this study, cellulose acetate/polyvinylpyrrolidone/ε-polylysine(CA/PVP/ε-PL) antibacterial nanofibrous membrane was prepared by electrospinning technology, and its structure and property were also characterized. The results showed that the fiber morphology was greatly influenced by the viscosity and conductivity of electrospinning solution, and the optimized ratio of CA, PVP and ε-PL were found to be 70, 35 and 20 g/L, under which, the diameter was uniformly distributed between 150~250 nm. It was indicated that ε-PL was successfully encapsulated in the fiber and there were strong interaction among different components by the results of fourier transform infrared spectroscopy, x-ray diffraction and differential scanning calorimeter. Thermogravimetric analysis revealed that the nanofiber had stronger thermal stabilities than pure components as the thermal decomposition temperature rised to be 441.30 ℃. Antimicrobial test demonstrated that the proposed nanofibrous membrane exhibited better antibacterial activity than casting film against Escherichia coli and Staphylococcus aureus with the inhibition zone diameter/ membrane mass ranging from 0.53 cm/mg to 0.67 cm/mg, furthermore, it can effectively prolong the storage time of fresh meat by more than 3 days. In conclusion, CA/PVP/ε-PL nanofibrous membrane possesses promising potential for antimicrobial packaging and exert a preservation effect on fresh meat, suggesting its potential for antimicrobial packaging. The study can aslo provide?a?theoretical?basis?for?the?application?of ε-PL-loaded?antibacterial?nanofibrous membrane.

国家自然科学基金项目;华南农业大学大学生创新创业训练计划项目