1-MCP处理维持软枣猕猴桃活性氧代谢平衡

1-MCP Treatment Maintained the Balance of Active Oxygen Metabolism in Actinidia Arguta

摘要:

为了明确1-MCP处理对软枣猕猴桃冰温（-0.5±0.3℃）贮藏0~60 d期间活性氧(ROS)代谢的影响，以“龙成二号”为研究试材，采用1.0 μL/L 1-甲基环丙烯（1-MCP)进行熏蒸处理，探究其对果实生理、活性氧代谢、抗坏血酸-谷胱甘肽循环的影响。结果表明：在贮藏60 d时1-MCP处理组果实呼吸强度为44.11 mg/(kg·h)，乙烯生成速率为2.35 μL/(kg·h)，分别比CK降低17.20%和23.70%，同时抑制超氧阴离子（O2-.）、过氧化氢（H2O2）、丙二醛(MDA)含量和相对电导率的升高，维持超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性，保持较高的还原型抗坏血酸(AsA)、氧化型谷胱甘肽(GSSG)和还原型谷胱甘肽(GSH)含量。1-MCP处理可以降低软枣猕猴桃的呼吸及乙烯生成速率，减少自由基生成，提高贮藏期的抗氧化能力，延缓果实衰老进程，保持良好的商品性，因此，本研究可以为软枣猕猴桃采后贮藏保鲜技术的完善提供理论基础和技术依据。

Abstract:

In order to determine the effect of 1-MCP treatment on the metabolism of reactive oxygen species (ROS) during 0 to 60 days of freezing temperature storage (-0.5±0.3℃), "Longcheng NO.2" was used as experimental material, and treated with 1.0 μL/ of 1-methylcyclopropene (1-MCP). The effects of 1-MCP on fruit physiology, active oxygen metabolism and ascorbate-glutathione cycle were analyzed. The results showed that after 60 days of storage, the respiration intensity and ethylene production rate of 1-MCP group were 44.11 mg/(kg·h) and 2.35 μL/(kg·h), which were 17.20% and 23.70% lower than those of CK, respectively. The increase of superoxide anion (O2-.), hydrogen peroxide (H2O2), malondialdehyde (MDA) content and relative conductivity in the fruit were inhibited. The activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and the contents of reduced ascorbic acid (AsA), oxidized glutathione (GSSG) and reduced glutathione (GSH) were maintained. 1-MCP treatment can reduce the respiration rate and ethylene production rate of Actinidia arguta, reduce the generation of free radicals, improve the antioxidant capacity during storage, delay the aging process of fruit, and maintain good commercial property. Therefore, this study can provide theoretical basis and technical basis for the improvement of postharvest storage and preservation technology of Actinidia arguta .