Abstract:In this study, 453 batches of shrimp imported from Vietnam, Thailand and Malaysia during 2017-2018 were selected to isolate and identify the pathogens of Acute Hepatopancreatic Necrosis Disease (AHPND). The results showed that the detection rate of the pathogen isolated from the hepatopancreas was only 2.87%, while the detection rate reached 16.11% after enrichment culture. Shrimp samples from Vietnam had the highest positive detection rate. The positive detection rate of 312 batches of samples was 21.79%, 11 positive strains were isolated, and the success rate of separation was 15.07%. In order to quickly, sensitively and reliably identify AHPND pathogens, a loop-mediated isothermal amplification (LAMP) method was established for rapid detection of AHPND pathogens. The results showed that only AHPND pathogen had obvious amplification curve and color change, the limit of plasmid detection was 1 fg/μL, which was 100 times higher than the conventional PCR method, and had strong stability. In 37 samples, the positive rate was 35.13%, which was higher than that of conventional PCR. The LAMP rapid detection method established in this study was suitable for specific detection of AHPND pathogens, and the isolation and identification analysis of pathogens could also provide reference data for laboratory detection of imported shrimps.