[关键词]
[摘要]
本文探究了LOX氧化体系下桑椹多酚(MP)及桑椹多酚-β-环糊精微胶囊(MPM)对猪肉肌原纤维蛋白结构与功能特性的影响。分别向肌原纤维蛋白中添加桑椹多酚、β-环糊精(β-CD)和桑椹多酚-β-环糊精微胶囊,经LOX氧化体系氧化后,分析桑椹多酚及其微胶囊对肌原纤维蛋白的结构和凝胶性能的影响。桑椹多酚可以有效抑制氧化过程中肌原纤维蛋白的羰基化、减少巯基向二硫键的转化,改善凝胶持水性。与氧化组相比,经过桑椹多酚处理后,羰基含量下降了33.33%,巯基含量提高了54.87%,二硫键含量下降了19.38%,使蛋白质的凝胶持水性从20.22%提高到了23.11%(p<0.05),但经桑椹多酚修饰后的肌原纤维蛋白热稳定性下降;β-CD对桑椹多酚进行包埋处理可减弱桑椹多酚对肌原纤维蛋白凝胶白度的负面影响,并通过强化蛋白质间的氢键作用进一步改善凝胶结构和持水性。以上研究表明,以β-环糊精为壁材制备桑椹多酚微胶囊有助于改善肌原纤维蛋白的氧化稳定性和凝胶结构,有望作为一种蛋白质氧化调节剂用于肉制品加工。
[Key word]
[Abstract]
The effect of mulberry polyphenols (MP) and mulberry polyphenols-β-CD microcapsule (MPM) on the structure and functional properties of pork myofibrillar protein under LOX oxidation system was investigated. Mulberry polyphenols, β-cyclodextrin (β-CD) and MPM were added into myofibrillar protein, respectively. After being oxidized by LOX oxidation system, the effects of mulberry polyphenols and their microcapsules on the structure and gel properties of myofibrillar protein were analyzed. The results suggested that MP could effectively inhibit the carbonylation of myofibrillar protein, reduce the transformation of sulfhydryl group to disulfide bond, and improve the water holding capacity of protein gel. Compared to the oxidation group, after being treated with MP, the carbonyl content decreased by 33.33%, the sulphydryl content increased by 54.87%, and the disulfide bond content decreased by 19.38%. The water holding capacity of the gel improved from 20.22% to 23.11% (p<0.05), but the thermal stability of myofibrillar protein was reduced with MP added. The negative effect of MP on the whiteness of gel could be weakened by β-CD embedding treatment, and the structure and water holding capacity of the gel could be improved by strengthening the hydrogen bond between proteins. These above results indicated that the mulberry polyphenol microcapsules made with β-CD was helpful to improve the oxidative stability and gel structure of myofibrillar protein, which was expected to be used as a protein oxidation regulator for meat processing.
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[基金项目]
广东省基础与应用基础研究基金项目(2019B1515210018);清远市科技计划项目(DZXQY031);广州市科技计划项目(201807010080)