本文以脑心浸液（BHI）肉汤为营养组分，以黄原胶与卡拉胶两种冷水可溶凝胶作为复合凝胶，研制出可应用于食品微生物菌落总数检测的凝胶型测试片。通过正交试验确定测试片培养基附加组分最优添加组合，并试验了两种凝胶不同复配比例对理化及微生物结果的影响，最终确定测试片培养基组分为BHI 38.5 g/L，TTC 0.008 g/L，SAP 0.05 g/L，丙酮酸钠2.0 g/L，且黄原胶与卡拉胶比例为8:2时，测试片达到最优性能。本文研制的菌落总数测试片，与国标平板计数方法相比，两种方法线性相关性达到0.99以上，测试片检出限可达2 CFU/mL，灵敏度达到100%；与行业主流产品3M Petrifilm菌落总数测试片相比，定量结果无明显差异，R2达到0.99。本文所研制的凝胶型菌落总数测试片可应用于食品中菌落总数的检测。

Brain heart infusion (BHI) broth as nutrient components and two cold water soluble gel, xanthan gum and carrageenan as complex gel were used to develop a gel bacterial testing plate for the detection of aerobic bacterial count in food. The optimal combination of the key additional components was determined by orthogonal test, and the effects of the ratio of the two gels on physical and chemical and microbiological results were examined. The optimal results were obtained when the components of medium were BHI 38.5 g/L, TTC 0.008 g/L, SAP 0.05 g/L, sodium pyruvate 1.5 g/L, and the proportion of complex gel was xanthan gum: carrageenan=8:2. Compared with national standard method, the gel testing plate method of this study had a good correlation (R2>0.99), achieving the same limit (2 CFU/mL) detection and sensitivity (100%); Compared with 3M Petrifilm, the quantitative results showed no significant difference, the two methods linear relationship was good (R2=0.99). The detection limit, sensitivity, and accuracy of the colony count testing plate in this study were no significant differences in comparison with the national method, it can be applied to the detection of colony count in food.

中国检验检疫科学研究院基本科研业务费专项资金资助项目（2018JK044）