本研究以黑果腺肋花楸为原料，β-环糊精为提取介质，探究了β-环糊精辅助提取黑果腺肋花楸中多酚类物质的可行性，并对β-环糊精所提取的多酚物质进行了分析。本研究在单因素试验的基础上，选取β-环糊精提取的最佳浓度、提取温度和提取时间进行三因素三水平Box-Behnken响应面试验，应用Design-Expert 8.0.6软件得到最佳提取条件，并对最佳提取条件的多酚类物质进行HPCL分析。结果表明：黑果腺肋花楸中多酚类物质的最佳条件为提取温度66 ℃、提取时间93 min、β-环糊精浓度33 g/L，在该条件下，多酚提取量为12.93 mg/g。对β-环糊精辅助提取所得黑果腺肋花楸多酚提取液进行HPLC分析，结果显示提取液中含有的多酚种类有香草酸、表儿茶素、阿魏酸、绿原酸及咖啡酸，它们的含量分别为239.80 μg/g、613.00 μg/g、17.48 μg/g、30.93 μg/g和16.11 μg/g。本研究表明β-环糊精可辅助提取黑果腺肋花楸中多酚类物质，为黑果腺肋花楸的开发与应用提供理论借鉴。

In this study, Aronia melanocarpa was used as the raw material, β-cyclodextrin was used as the extraction medium, and the feasibility of β-cyclodextrin-assisted extraction of polyphenols from Aronia melanocarpa was explored. On the basis of single factor experiments, the optimal concentration of β-cyclodextrin, extraction temperature and extraction time were selected for the three-factor three-level Box-Behnken response surface test, and the Design-Expert 8.0.6 software was used to obtain the optimal extraction conditions. HPCL analysis of the polyphenols obtained under the optimal extraction conditions was performed. The results showed that the optimal conditions for extracting the polyphenols in Aronia melanocarpa were: temperature, 66 ℃; extraction duration, 93 min; β-cyclodextrin concentration, 33 g/L. Under these conditions, the amount of extracted polyphenols was 12.93 mg/g. The HPLC analysis of the polyphenol extract of Aronia melanocarpa obtained by β-cyclodextrin-assisted extraction showed that the polyphenols contained in the extract were vanillic acid, epicatechin, ferulic acid, chlorogenic acid and caffeic acid, with their contents were as 239.80 μg/g, 613.00 μg/g, 17.48 μg/g, 30.93 μg/g, and 16.11 μg/g, respectively. This research shows that β-cyclodextrin can assist the extraction of polyphenols from Aronia melanocarpa, which provides a theoretical reference for the development and application of Aronia melanocarpa.

陕西省重点研发计划项目（2019NY－107）