本研究了灵芝多糖对人肺癌A549细胞增殖凋亡的作用。将肺癌A549细胞分为空白组、药物对照组、低、中、高浓度组，对各组细胞分别进行干预，检测其凋亡、增殖能力变化情况，对细胞凋亡蛋白Bcl-2、PI3K、Akt表达进行检测。结果显示，相比其他各组，高浓度组细胞增殖率较低，且随着使用灵芝多糖干预时间延长，高浓度组细胞增殖率自44.37%下降至20.11%。相比其他各组，高浓度组细胞凋亡率较高，且随着使用灵芝多糖干预时间延长，高浓度组细胞凋亡率自46.52%上升至77.93%。相比其他各组，高浓度组细胞Bcl-2、PI3K、Akt相对表达量较低。使用灵芝多糖进行干预，能够抑制肺癌A549细胞增殖，促进肺癌A549细胞凋亡，其能力与自身细胞毒性无关，可能与调控细胞凋亡相关蛋白Bcl-2、PI3K、Akt表达有关，说明灵芝多糖能够抑制肺癌A549细胞发展和扩散，对人肺癌A549细胞增殖凋亡起到调控。

In this study, the effects of Ganoderma lucidum polysaccharide on the proliferation and apoptosis of human lung cancer A549 cells were investigated. Lung cancer A549 cells were divided into a blank group, drug control group, and low-, medium- and high-concentration groups. The cells in each group were intervened to detect the changes in apoptosis and proliferation ability, as well as the expression of apoptotic proteins Bcl-2, PI3K and Akt. The results showed that, compared with other groups, the high-concentration group had a lower cell proliferation rate, and its cell proliferation rate decreased from 44.37% to 20.11% when the time of intervention with Ganoderma lucidum polysaccharide was extended. Compared with other groups, the high-concentration group had a higher apoptosis rate, and its apoptosis rate increased from 46.52% to 77.93% when the time of intervention with Ganoderma lucidum polysaccharide was extended. Compared with other groups, the high-concentration group had lower relative expression levels of Bcl-2, PI3K and Akt. The intervention with Ganoderma lucidum polysaccharide can inhibit the proliferation of lung cancer A549 cells and promote the apoptosis of lung cancer A549 cells. Its ability has nothing to do with its own cytotoxicity, but may be related to the regulation of the expression of apoptosis-related proteins, Bcl-2, PI3K and Akt. These results indicate that Ganoderma lucidum polysaccharide can inhibit the development and spread of lung cancer A549 cells and regulate the proliferation and apoptosis of human lung cancer A549 cells.

河北省沧州市科技计划项目（162302097）