[关键词]
[摘要]
本研究基于人体肠道微生态系统模拟装置(simulator of the human intestinal microbial ecosystem, SHIME),引入人体肠道微生物模拟人体体外微生态,通过16S rRNA高通量测序技术分析橘皮汤对肠道菌群的影响,通过气相色谱技术分析橘皮汤对肠道菌群代谢产物短链脂肪酸的影响。结果显示橘皮汤干预后韦荣氏球菌属水平相对丰度在干预期下降了33.65%、在维持期下降了92.78%,克雷伯菌属水平相对丰度在干预期下降了63.60%、在维持期下降了67.82%,巨单胞菌属水平相对丰度在干预期升高了27.5%、在维持期升高了62.61%;短链脂肪酸中乙酸含量先下降后上升,总体增加了3.21%,丙酸含量减少了45.43%(p<0.01),异丁酸、丁酸、异戊酸与戊酸的含量显著增加(p<0.01),分别增加了52.94%、40.86%、48.94%和80.00%。橘皮汤干预能起到改变肠道微生物多样性的功效,并具有调节菌群丰度水平的作用,表现出抑制韦荣氏球菌属、克雷伯菌属等肠道有害微生物的生长,并对肠道菌群代谢产物短链脂肪酸的产生有一定的促进作用,有效改善了人体肠道菌群结构。
[Key word]
[Abstract]
This study was based on the simulator of the human intestinal microbial ecosystem (SHIME), and human intestinal microorganisms were introduced to simulate in vitro the human microbial ecology. The effects of jupi decoction on intestinal flora were examined by 16S rRNA high-throughput sequencing technology, and the effects of jupi decoction on the metabolites of intestinal microflora, short-chain fatty acids (SCFAs), were investigated by gas chromatography. The results showed that after the intervention of jupi decoction, the relative abundance of Veronica decreased by 33.65% during the intervention period and by 92.78% during the maintenance period. The relative abundance of Klebsiella decreased by 63.60% and 67.82%, respectively, during the intervention and maintenance periods, while that of Macromonas increased by 27.5% and 62.61% during the intervention and maintenance periods, respectively. The content of acetic acid among the short chain fatty acids decreased first and then increased (by 3.21% in general), while the content of propanoic acid decreased by 45.43% (p<0.01). The contents of isobutyric acid, butyric acid, isovaleric acid and valeric acid increased by 52.94%, 40.86%, 48.94% and 80.00%, respectively. The intervention with jupi decoction can change the diversity of intestinal microflora, regulate the level of microbial abundance, exhibit inhibitory effects on the growth of harmful intestinal microorganisms such as Veronica and Klebsiella, and promote the production of intestinal microbial metabolites, short chain fatty acids, which improves effectively the structure of human intestinal flora.
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[基金项目]
国家自然科学基金项目(81773850);湖南省科技重大专项(2017SK1020);长沙市科技项目(kh1801123);湖南农业大学青年基金(19QN26)