通过建立D-半乳糖致衰老模型，观察恭城油茶对衰老小鼠氧化应激的影响。KM小鼠随机分成正常组、衰老模型组、Vc组（100 mg/kg）、恭城油茶组（750 mg/kg）。检测肝脏和肾脏指数，血清、肝脏和肾脏组织中谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）活性、总抗氧化能力（T-AOC）活力以及丙二醛（MDA）水平等指标；制备肝脏和肾脏切片，观察肝脏和肾脏组织病理变化情况。恭城油茶能显著改善D-半乳糖造成的肝脏和肾脏指数下降（p<0.05），油茶灌胃组肝脏、肾脏指数与衰老组比分别升高了11.87%和25.38%；明显提高衰老小鼠血清、肝脏和肾脏中GSH-Px、T-SOD酶活性和T-AOC活力，同时显著降低MDA含量（p<0.05）；与衰老组相比，油茶灌胃组肝脏中GSH-Px、T-SOD和T-AOC分别升高62.98%、98.35%和714.63%（p<0.05），且MDA含量降低了65.42%（p<0.05）；肾脏中GSH-Px、T-SOD和T-AOC的增加幅度分别为119.66%、182.81%和155.88%（p<0.05），MDA水平下降了74.61%（p<0.05）。切片观察结果显示，恭城油茶还可缓解衰老小鼠肝脏和肾脏损伤。上述结果表明，恭城油茶能通过减轻氧化应激来抑制小鼠衰老导致的氧化应激相关肝脏和肾脏器官的损伤。

Effect of Gongcheng oil tea on oxidative stress in D-galactose-induced aging mice was investigated. Healthy KM mice of reproductive age were randomly divided into 4 groups: normal control, aging model, Vc groups (100 mg/kg) and Gongcheng oil tea groups (750 mg/kg). The enzyme activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (T-SOD), as well as the total antioxidant capacity (T-AOC) and malondialdehyde (MDA) level in serum, liver and kidney tissue of mice were determined. The pathological changes of liver and kidney tissues were observed. Gongcheng oil tea could significantly improve the liver and kidney indexes of mice that decreased by D-galactose (p<0.05). Compared with those of the aging group, the liver and kidney indexes of the oil tea group increased by 11.87% and 25.38% respectively. Pretreatment with Gongcheng oil tea, the enzyme activities of T-SOD and GSH-Px, as well as T-AOC levels in serum, liver and kidney tissue of mice increased significantly, while MDA production in serum, liver and kidney tissue of mice decreased significantly (p<0.05). Compared with the senescence group, GSH-PX, T-SOD and T-AOC in the liver of oil tea group increased by 62.98%, 98.35% and 714.63%, respectively (p<0.05), and MDA decreased by 65.42% (p<0.05). The activities of GSH-Px, T-SOD and T-AOC in kidney increased by 119.66%, 182.81% and 155.88% (p<0.05), respectively, while MDA decreased by 74.61% (p<0.05). Pathological observation showed that Gongcheng oil tea could alleviate liver and kidney damage during aging. The results showed that Gongcheng oil tea could inhibite aging-induced oxidative stress-related liver and kidney injuries in mice by attenuating oxidative stress.

国家自然科学基金资助项目(31760530)；广西高等学校千名中青年骨干教师培育计划资助（桂教人[2018]18号）；广西自然科学基金(2014GXNSFBA118124；2016GXNSFAA380031)；桂林医学院科研启动基金项目(KY2012100)