以芹菜叶粉末为原料，芹菜素含量为指标，优化超声辅助低共熔溶剂提取芹菜叶中芹菜素的提取工艺。用筛选的最优低共熔溶剂进行单因素试验，考察含水量、液料比、超声时间、提取温度对芹菜素含量的影响。在单因素的基础上，利用Box-Behnken响应面优化提取条件。AB-8型大孔树脂纯化提取液后，利用紫外可见光谱和液质联用对芹菜素纯化物进行分析和验证。最后，研究了芹菜素纯化物的抗氧化性。结果表明：低共熔溶剂（氯化胆碱/乙醇）比80%乙醇提取芹菜素的效率高17.78%。最优提取条件为：液料比40:1；含水量24%；超声时间14 min；提取温度42 ℃。此条件下得到的芹菜素含量为16.87 mg/g。芹菜素纯化物对DPPH、ABTS自由基具有良好的清除能力，对OH自由基清除效果更好，其IC50分别为82.44、148.92和8.69 μg/mL，但均低于抗坏血酸和芹菜素标准品的清除能力。该绿色环保的低共熔溶剂能高效提取芹菜叶中芹菜素，且能够保持良好的抗氧化活性。

Using celery leaf powder as the raw material and apigenin content as the index, the extraction process of apigenin in celery leaves was optimized. The effects of water content, liquid-to-material ratio, ultrasonic time, and extraction temperature on the apigenin content were investigated in the best deep eutectic solvent. On the basis of single factor, the extraction conditions were optimized by response surface methodology. Using UV-visible spectroscopy and liquid chromatography-mass spectrometry (LC-MS) apigenin was analyzed and verified in the purified extract with macroporous resin. Finally, the antioxidant properties of purified apigenin were studied. The results showed that the efficiency of choline chloride/ethanol was 17.78% higher than that of 80% ethanol in the extraction of apigenin. The optimal conditions were as follow: liquid-to-material ratio of 40:1; water content of 24%; ultrasonic time of 14 min; extraction temperature of 42 ℃. Under these conditions, the apigenin content was 16.87 mg/g. The purified apigenin had a certain scavenging effect on DPPH (IC50=82.44 μg/mL) and ABTS (IC50=148.92μg/mL) free radicals and a strong scavenging ability on hydroxyl (IC50=8.69 μg/mL) free radicals, which were lower than the scavenging ability of ascorbic acid and apigenin standard products. The green and environment-friendly DES can be used to extract apigenin from celery leaves, and the purified apigenin can maintain good antioxidant activity.

湖北省高等学校优秀中青年科技创新团队计划项目（LT201911）；中央引导地方科技发展专项资金（鄂财教发[2017]88号)