刘琴,周游,陈轩,沈汪洋,庄坤,吕庆云.超声辅助低共熔溶剂提取芹菜叶中的芹菜素及其抗氧化性分析[J].,2021,37(3):202-211.
超声辅助低共熔溶剂提取芹菜叶中的芹菜素及其抗氧化性分析
Optimization of Ultrasonic Assisted Deep Eutectic Solvents Extraction Process and Antioxidant Activity of Apigenin from Celery Leaves
投稿时间:2020-09-01  
DOI:10.13982/j.mfst.1673-9078.2021.3.0821
中文关键词:  芹菜素  低共熔溶剂  响应面  超声辅助提取  抗氧化
英文关键词:apigenin  deep eutectic solvent  response surface methodology  ultrasonic-assisted extraction  antioxidant
作者简介:刘琴(1994-),女,硕士研究生,研究方向:谷物资源开发利用 通讯作者:吕庆云(1980-),男,博士,副教授,研究方向:谷物资源开发利用
基金项目:湖北省高等学校优秀中青年科技创新团队计划项目(LT201911);中央引导地方科技发展专项资金(鄂财教发[2017]88号)
作者单位
刘琴 (1.武汉轻工大学食品科学与工程学院,湖北武汉 430023) 
周游 (1.武汉轻工大学食品科学与工程学院,湖北武汉 430023) 
陈轩 (2.武汉轻工大学湖北省稻谷加工工程技术中心,大宗粮油精深加工教育部重点实验室,湖北武汉 430023) 
沈汪洋 (1.武汉轻工大学食品科学与工程学院,湖北武汉 430023) (2.武汉轻工大学湖北省稻谷加工工程技术中心,大宗粮油精深加工教育部重点实验室,湖北武汉 430023) 
庄坤 (2.武汉轻工大学湖北省稻谷加工工程技术中心,大宗粮油精深加工教育部重点实验室,湖北武汉 430023) 
吕庆云 (1.武汉轻工大学食品科学与工程学院,湖北武汉 430023) (2.武汉轻工大学湖北省稻谷加工工程技术中心,大宗粮油精深加工教育部重点实验室,湖北武汉 430023) 
AuthorInstitution
LIU Qin (1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China) 
ZHOU You (1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China) 
CHEN Xuan (2.Hubei Rice Processing Engineering Technology Center, Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China) 
SHEN Wang-yang (1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China) (2.Hubei Rice Processing Engineering Technology Center, Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China) 
ZHUANG Kun (2.Hubei Rice Processing Engineering Technology Center, Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China) 
LYU Qing-yun (1.College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China) (2.Hubei Rice Processing Engineering Technology Center, Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China) 
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中文摘要:
      以芹菜叶粉末为原料,芹菜素含量为指标,优化超声辅助低共熔溶剂提取芹菜叶中芹菜素的提取工艺。用筛选的最优低共熔溶剂进行单因素试验,考察含水量、液料比、超声时间、提取温度对芹菜素含量的影响。在单因素的基础上,利用Box-Behnken响应面优化提取条件。AB-8型大孔树脂纯化提取液后,利用紫外可见光谱和液质联用对芹菜素纯化物进行分析和验证。最后,研究了芹菜素纯化物的抗氧化性。结果表明:低共熔溶剂(氯化胆碱/乙醇)比80%乙醇提取芹菜素的效率高17.78%。最优提取条件为:液料比40:1;含水量24%;超声时间14 min;提取温度42 ℃。此条件下得到的芹菜素含量为16.87 mg/g。芹菜素纯化物对DPPH、ABTS自由基具有良好的清除能力,对OH自由基清除效果更好,其IC50分别为82.44、148.92和8.69 μg/mL,但均低于抗坏血酸和芹菜素标准品的清除能力。该绿色环保的低共熔溶剂能高效提取芹菜叶中芹菜素,且能够保持良好的抗氧化活性。
英文摘要:
      Using celery leaf powder as the raw material and apigenin content as the index, the extraction process of apigenin in celery leaves was optimized. The effects of water content, liquid-to-material ratio, ultrasonic time, and extraction temperature on the apigenin content were investigated in the best deep eutectic solvent. On the basis of single factor, the extraction conditions were optimized by response surface methodology. Using UV-visible spectroscopy and liquid chromatography-mass spectrometry (LC-MS) apigenin was analyzed and verified in the purified extract with macroporous resin. Finally, the antioxidant properties of purified apigenin were studied. The results showed that the efficiency of choline chloride/ethanol was 17.78% higher than that of 80% ethanol in the extraction of apigenin. The optimal conditions were as follow: liquid-to-material ratio of 40:1; water content of 24%; ultrasonic time of 14 min; extraction temperature of 42 ℃. Under these conditions, the apigenin content was 16.87 mg/g. The purified apigenin had a certain scavenging effect on DPPH (IC50=82.44 μg/mL) and ABTS (IC50=148.92μg/mL) free radicals and a strong scavenging ability on hydroxyl (IC50=8.69 μg/mL) free radicals, which were lower than the scavenging ability of ascorbic acid and apigenin standard products. The green and environment-friendly DES can be used to extract apigenin from celery leaves, and the purified apigenin can maintain good antioxidant activity.
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