张想,李立郎,杨娟,杨小生,王瑜,王丽,廖秀,李全力,李齐激,王道平,杨艳,李春燕,葛丽娟.茶树菇发酵刺梨果渣制备可溶性膳食纤维工艺优化及其对小鼠润肠通便功能的评价[J].,2021,37(3):171-180.
茶树菇发酵刺梨果渣制备可溶性膳食纤维工艺优化及其对小鼠润肠通便功能的评价
Process Optimization of Soluble Dietary Fiber from Roxburgh rose Pomace Fermented by Agrocybe aegerita and Its Evaluation on the Moistening and Laxative Function of Mice
投稿时间:2020-09-11  
DOI:10.13982/j.mfst.1673-9078.2021.03.0852
中文关键词:  刺梨果渣  可溶性膳食纤维  茶树菇发酵  响应面法  润肠通便
英文关键词:Roxburgh rose pomace  soluble dietary fiber  Agrocybe aegerita fermentation  response surface methodology  moistening and laxative
作者简介:张想(1995-),女,硕士,研究方向:食品科学与工程 通讯作者:李立郎(1987-),男,助理研究员,研究方向:健康养生产品开发
基金项目:贵州省科技支撑项目(黔科合支撑[2018]2769);贵州省科技平台及人才团队计划项目(黔科合平台人才[2017]5614:黔教合KY字(2020)018);中央和省级农业生产专项资金(ZS202020)
作者单位
张想 (1.贵州大学酿酒与食品工程学院,贵州贵阳 550025) 
李立郎 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
杨娟 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
杨小生 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
王瑜 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
王丽 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
廖秀 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
李全力 (3.桐梓县官仓镇农业服务中心,贵州遵义 563200) 
李齐激 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
王道平 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
杨艳 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
李春燕 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
葛丽娟 (2.贵州省中国科学院天然产物化学重点实验室,贵州贵阳 550014) 
AuthorInstitution
ZHANG Xiang (1.College of Wine and Food Engineering Guizhou University, Guiyang 550025, China) 
LI Li-lang (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
YANG Juan (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
YANG Xiao-sheng (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
WANG Yu (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
WANG Li (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
LIAO Xiu (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
LI Quan-li (3.Tongzi County Guancang Town Agricultural Service Center, Zunyi 563200, China) 
LI Qi-ji (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
WANG Dao-ping (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
YANG Yan (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
LI Chun-yan (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
GE Li-juan (2.Key Laboratory of Natural Product Chemistry Chinese Academy of Sciences Guizhou Province, Guiyang 550014, China) 
摘要点击次数: 15
全文下载次数: 15
中文摘要:
      通过食用菌茶树菇发酵刺梨果渣,提高可溶性膳食纤维(SDF)含量,探究制备所得刺梨果渣SDF的润肠通便功能,为综合开发利用刺梨果渣膳食纤维提供依据。方法:在单因素的基础上,根据Box-Behnken实验设计对发酵培养基配方进行优化以提高SDF得率;将KM小鼠随机分为空白对照组、模型对照组、阳性对照组、SDF低(ig.0.25 g/kg?bw)、中(ig.0.50 g/kg?bw)、高剂量组(ig.1 g/kg?bw),以复方地芬诺酯建立便秘模型,连续灌胃14 d后通过测定小肠墨汁推进率、首粒黑便排出时间、6 h内排黑便粒数及重量,分析其对小鼠润肠通便功能的影响。结果:最佳制备工艺条件为氯化钙添加量1%、氯化钾添加量0.60%、糖添加量4%、液料比7:1;在此条件下刺梨果渣SDF得率为5.86%,较比未发酵原果渣SDF提高了68.78%。动物实验结果显示,在便秘模型构建成功的基础上,SDF各剂量组均有改善小鼠便秘情况的作用,其中高剂量组作用效果最明显。结论:采用食用菌茶树菇发酵刺梨果渣SDF的制备工艺合理、经济简便,所得产品具有良好的润肠通便功能。
英文摘要:
      Rosa roxburghii pomace was fermented by Agrocybe aegerita to increase the content of soluble dietary fiber (SDF). The function of moistening intestines and defecation of Rosa roxburghii pomace was also investigated, which could provide the basis for the comprehensive development and utilization of dietary fiber of Rosa roxburghii pomace. Methods: on the basis of single factor and Box-Behnken experimental design, the fermentation medium was optimized to improve the yield of SDF. KM mice were randomly divided into blank control group, model control group, positive control group, SDF low (ig. 0.25 g/kg?bw), medium (ig. 0.5 g/kg?bw) and high-dose group (ig. 1 g/kg?bw). The constipation model was established with compound diphenoll. After continuous intragastric administration for 14 days, the effect of the intragastric infusion on the moistening and laxative function of mice was analyzed by measuring the propulsion rate of small intestine ink, the time of the first black stool, and the number and weight of the first black stool within 6 h. Results: the optimum preparation conditions were as follows: calcium chloride of 1%, potassium chloride of 0.6%, sugar of 4% and ratio of liquid to material of 7:1. Under these conditions, the SDF yield of Rosa roxburghii pomace was 5.86%, which was 68.78% higher than that of unfermented pomace. The results of animal experiments showed that on the basis of successful constipation model, all SDF dose groups could improve the constipation of mice, and the effect of high dose group was the most obvious. Conclusion: the preparation technology of SDF from Rosa roxburghii pomace fermented by Agrocybe aegerita was reasonable, economic and convenient, and the product could have a good effect of moistening and laxative.
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