王日明,宁雪,任跃英.黄芪甲苷抑制黑色素瘤B16细胞的增殖作用[J].,2021,37(3):32-36.
黄芪甲苷抑制黑色素瘤B16细胞的增殖作用
Astragaloside IV Inhibits Proliferation and Induces Apoptosis of Melanoma B16 Cells
投稿时间:2020-09-14  
DOI:10.13982/j.mfst.1673-9078.2021.3.0860
中文关键词:  黄芪甲苷  黑色素瘤  增殖  凋亡
英文关键词:astragaloside IV  melanoma  proliferation  apoptosis
作者简介:王日明(1994-),男,硕士,研究方向:中药炮制及药理 通讯作者:任跃英(1958-),女,博士,教授,研究方向:中药栽培及育种
基金项目:吉林省科技发展计划项目(2018020101)
作者单位
王日明 (吉林农业大学中药材学院,吉林长春 130118) 
宁雪 (吉林农业大学中药材学院,吉林长春 130118) 
任跃英 (吉林农业大学中药材学院,吉林长春 130118) 
AuthorInstitution
WANG Ri-ming (Jilin Agricultural University College of Traditional Chinese Medicine, Changchun 130118, China) 
NING Xue (Jilin Agricultural University College of Traditional Chinese Medicine, Changchun 130118, China) 
REN Yue-ying (Jilin Agricultural University College of Traditional Chinese Medicine, Changchun 130118, China) 
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中文摘要:
      本文研究了黄芪甲苷抑制黑色素瘤B16细胞增殖并诱导细胞凋亡的影响。将细胞培养成功后,分别分成空白对照组、低浓度组(10 mg/mL)、中浓度组(20 mg/mL)、高浓度组(50 mg/mL),检测黄芪甲苷对黑色素瘤B16细胞增殖及凋亡的影响,并测定Caspase-3、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、Bax、β-catenin等蛋白的表达量。结果表明,黄芪甲苷具有较好的抗氧化活性,50 mg/mL处理时,其还原能力和DPPH自由基清除能力分别为0.92和70.82%;在此作用浓度下,处理72 h后,其对B16细胞的增殖率和凋亡率分别为15.55%和40.45%,和其他各组均有显著性差异(p<0.05)。此外,在高浓度黄芪甲苷作用下,Caspase-3、Bcl-2、Bax、β-catenin蛋白的表达量分别为0.56、0.58、0.27、0.25,显著区别于其他各实验组(p<0.05),说明黄芪甲苷能抑制较好黑色素瘤B16细胞的增殖,并通过调控Caspase-3、Bcl-2、Bax、β-catenin蛋白表达,诱导细胞凋亡。
英文摘要:
      The effect of astragaloside IV on the proliferation and apoptosis of melanoma B16 cells was investigated. After the cells were cultured successfully, they were divided into blank control group, low concentration group (10 mg/mL), medium concentration group (20 mg/mL) and high concentration group (50 mg/mL). The effects of astragaloside IV on proliferation and apoptosis of melanoma B16 cells were evaluated, and the expression levels of caspase-3, B-cell lymphoma-2 (Bcl-2), bax and β-catenin were detected. The results showed that astragaloside IV had good antioxidant activity. When treated with 50 mg/mL, the reducing ability and DPPH free radical scavenging capacity were 0.92 and 70.82%, respectively. Under this concentration, the proliferation rate and apoptosis rate of B16 cells after 72 h treatment were 15.55% and 40.45%, respectively, which were significantly different from those of other groups (p<0.05). In addition, the expression levels of caspase-3, Bcl-2, bax and β-catenin were 0.56, 0.58, 0.27 and 0.25, respectively, which were significantly different from those of other groups (p<0.05). Results indicated that astragaloside IV could inhibit the proliferation of melanoma B16 cells and induce apoptosis by regulating the expression of caspase-3, Bcl-2, bax and β-catenin.
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