研究构树叶总黄酮的最佳提取工艺及主要成分组成，采用单因素试验和响应面设计优化分析乙醇浓度、提取时间、提取温度以及料液比对总黄酮得率的影响，同时测定提取液中总黄酮的组成。结果表明，当乙醇浓度30%、提取时间2.5 h、温度69 ℃、料液比9%时，构树叶总黄酮的最高得率为37.33 mg/g；利用超高液相色谱-串联质谱（UPLC-MS/MS）平台进行成分分析，鉴定出的黄酮类化学物共有11种，分别为：野黄芩苷、荭草苷、芹黄素-7-O-葡萄醛酸苷、山奈酚、芹菜素、木犀草苷、高良姜素、芹菜素-7-葡萄糖苷、牡荆素葡萄糖苷、6-O-对羟基苯甲酰基桃叶珊瑚苷、牡荆素鼠李糖苷。采用响应面法的Box-Behken设计优化醇法提取构树叶总黄酮，确定了构树叶中总黄酮最佳提取工艺，该工艺简单易行、稳定性好，能够为构树叶黄酮的工业化生产提供指导和参考。

In order to investigate the optimal extraction process and the main components of flavonoids from the leaves of Broussonetia papyrifera, single factor experiments and response surface methodology (RSM) analysis were conducted to examine the effects of ethanol concentration, extraction time, extraction temperature and liquid-material ratio on the yield of flavonoids, as well as the flavonoid composition of the extract. The results showed that when the ethanol concentration was 30%, extraction time was 2.5 h, the extraction temperature was 69 ℃, and liquid-material ratio was 9%, the yield of flavonoids in Broussonetia papyrifera leaves was 37.33 mg/g; UPLC-MS (UPLC-MS/MS) was used for component analysis, a total of 11 species of flavonoids were identified: scutellarin, orientin, apigenin-7-O-glucuronide, kaempferol, apigenin, cynaroside, galangin, apigenin-7-glucoside, (1ξ)-1,5-anhydro-1-(5,7-dihydroxy-4-oxo-2-phenyl-4h-chromen-8-yl)-d-glucitol, 6-O-phydroxybenzoylaucubin, and isovitexin. The Box-Behnken Design of response surface methodology was used to optimize the ethanolic extraction of total flavonoids form Broussonetia papyrifera leaves, while determiningthe best extraction process for the total flavonoids. The process was simple and easy to implementwith good stability, which can provide guidance and +referencefor the industrial production of flavonoids from Broussonetia papyrifera leaves.

国家重点研发计划项目(2016YFD0400500)；济南市科技发展计划（201816003）