本文研究葛根素提取物对妊娠期糖尿病模型大鼠氧化应激的作用。建立妊娠期糖尿病模型大鼠，采用葛根素提取物进行干预。检测大鼠空腹血糖（FPG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）、丙二醛（MDA）、一氧化氮（NO）、超氧化物歧化酶（SOD）、白介素-6（IL-6）、C反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α），醌氧化还原酶1（NQO1）、血红素氧合酶-1（HO-1）及核因子E2相关因子2（Nuclear factor E2 related factor 2）Nrf2蛋白的含量水平。结果表明，葛根素高剂量组FPG、LDL-C和HDL-C分别为9.05 mmol/L、0.79 mmol/L和0.72 mmol/L（p<0.05）；葛根素高剂量组MDA、NO和SOD分别为7.15 nmol/mg、90.76 μmol/L和9.29 U/mg（p<0.05）；葛根素高剂量组IL-6、CRP和TNF-α分别为135.51 ng/L、7.18 mg/L和15.06 μg/μL（p<0.05）。结果说明葛根素提取物可降低空腹血糖，改善MDA、NO及SOD水平。

The effect of puerarin extract on oxidative stress in gestational diabetes model rats was studied. Gestational diabetes model rats were established and puerarin extract was used for intervention. Fasting blood glucose (FPG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), interleukin-6 (IL-6), C-reactive protein (CRP), tumor necrosis factor (TNF-α), Quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1) and nuclear factor E2 related factor 2 (Nrf2) proteins were detected. Results were showed that the FPG, LDL-C and HDL-C of high dose puerarin group were 9.05 mmol/L, 0.79 mmol/L and 0.72 mmol/L respectively, which were significantly different with other groups (p<0.05). The MDA, NO and SOD were 7.15 nmol/mg, 90.76 μmol/L and 9.29 U/mg respectively, The levels of IL-6, CRP and TNF-α in puerarin high-dose group were 135.51 ng/L, 7.18 mg/L and 15.06 μg/μL respectively, which were lower than those in model groups (p<0.05). Puerarin extract can reduce fasting blood glucose and improve MDA, NO and SOD levels.

石家庄市科学技术研究与发展项目（181461483）