本文旨在研究药食两用中药党参、茯苓、甘草水提物对学习记忆障碍小鼠的改善作用，评价其抗氧化活性及免疫调节功效。腹腔注射氢溴酸东莨菪碱建立学习记忆障碍模型，新物体识别实验评价水提物对学习记忆障碍小鼠的行为学改善作用；ELISA检测小鼠脑海马组织中SOD、GSH-Px酶活性及MDA含量，血清IL-2、TNF-α水平，脾脏NK细胞活性。负重游泳实验评价水提物对正常小鼠的抗疲劳作用；比重法测定胸腺指数与脾脏指数。结果发现，水提物高剂量组（40 g/kg·d）的效果最佳，在不影响小鼠自主活动的前提下，能够使记忆障碍小鼠的新物体识别指数提高18.35%；SOD、GSH-Px分别提高了65.56 U/mg、437.00 U/mg，MDA降低了6.30 nmol/mg；IL-2、TNF-α分别降低了78.43 pg/mL、160.20 pg/mL，NK细胞的活性提高了2488.81 U/g。还能够使正常小鼠的负重游泳时间比正常组增加60.12 s；胸腺指数与脾脏指数分别增加了0.06%与0.10%，SOD、GSH-Px分别提高了79.52 U/mg、345.30 U/mg，MDA降低了1.05 nmol/mg，NK细胞活性提高了1816.31 U/g。这表明党参-茯苓-甘草水提物能够发挥其抗氧化、免疫调节功能，对学习记忆障碍小鼠起到改善作用，并且具有一定抗疲劳功效。

To study the effects of traditional Chinese medicine Radix codonopsis, poria cocos and Radix glycyrrhizae water extract on learning and memory impaired mice, the antioxidant and immunomodulatory effects of water extract on learning and memory impaired mice and normal mice were evaluated. Intraperitoneal injection of scopolamine hydrobromide was used to model learning and memory impairment. ELISA was used to detect SOD, GSH-Px enzyme activity and MDA content in hippocampus of mice, serum IL-2, TNF-α level, and spleen NK cell activity. The anti-fatigue effect of water extract on normal mice was evaluated in weight-bearing swimming experiment. Thymus index and spleen index were determined by gravity method. The results showed that the high dose of water extract (40 g/kg·d) had the best effect, and the novel object recognition index of memory impaired mice could be increased by 18.35% without affecting the independent activity of mice. SOD and GSH-Px increased by 65.56 U/mg, 437.00 U/mg, and MDA decreased by 6.30 nmol/mg. IL-2 and TNF-α decreased by 78.43 pg/mL, 160.20 pg/mL, and the activity of NK cell increased by 2488.81 U/g. It was also possible to increase the weight swimming time of normal mice by 60.12 s compared with the normal group. Thymus index and spleen index increased by 0.06% and 0.10%; SOD and GSH-Px increased by 79.52 U/mg and 345.30 U/mg, respectively, MDA decreased by 1.05 nmol/mg, and NK cell activity increased by 1816.31 U/g. The results showed that the water extract of Radix Codonopsis-Poria cocos-Glycyrrhizae Radix could improve the learning and memory impairment of mice and exert anti-fatigue effect on normal mice, its mechanism was related to its anti-oxidation and immunomodulatory function.

国家自然科学基金资助项目（81473375）；山西省重点研发计划（国际科技合作）重大区域创新合作项目（201803D421006；201903D421018）；山西省回国留学人员科研资助项目（2013-134）；晋药综合开发利用协同创新中心项目（2018-JYXT-05）