以纹党多糖（Codonopisis pilosula polysaccharide，CPP）为材料，研究多糖对黑腹果蝇（Drosophila melanogaster）生育力和寿命及抗氧化活力的影响。将黑腹果蝇随机分为50、100、200和400 mg/L剂量处理组，以生理盐水为对照组，连续培养四代，考查不同浓度的纹党多糖对果蝇生存能力的影响，包括果蝇子一代到子四代（F1~F4）生育力和寿命的变化，雌、雄果蝇数量的变化，测定百草枯急性氧化损伤下的存活时间、抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活力及丙二醛（MDA）含量的变化。结果显示，400 mg/L剂量的CPP能显著提高F1~F4代雌、雄果蝇世代生育力，与对照相比后代果蝇数量增加了150%（p<0.01），延长F1~F4代雌、雄果蝇寿命，与对照相比后代果蝇寿命增加了34.07%（p<0.01），延长百草枯急性氧化损伤下F1~F4代雌、雄果蝇的平均存活时间，与对照相比存活时间延长了7.06 h和6.28 h（p<0.01），同时提高了果蝇体内SOD和CAT活性（p<0.01），降低了果蝇体内的MDA含量（p<0.01）。研究结果表明，CPP能显著提高F1~F4代雌、雄果蝇世代生育力，延长寿命，延长百草枯急性氧化损伤下果蝇的存活时间，提高了果蝇的抗氧化能力和生命力，促进了生殖能力，表现出剂量效应，果蝇世代总量雌、雄果性别比接近1:1。

In order to evaluate the effect of Codonopisis pilosula polysaccharides on the reproduction capability and life span and antioxidant capacity of Drosophila melanogaster, survival experiments were conducted to examine the influences of different concentrations (50, 100, 200, 400 mg/L) of Codonopisis pilosula polysaccharides on the reproduction capability and life span of four generations (F1 to F4), the number of female and male, the survival time in paraquat treatment, and the activity of antioxidant enzyme superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) contents. The results showed that the 400 mg/L dose of CPP could significantly increase the fertility of F1~F4 generations female and male Drosophila generations. Compared with the control, the number of offspring Drosophila generations increased by 150% (p<0.01), and extended the life span of F1 to F4 female and male Drosophila generations. The lifespan of offspring Drosophila generations increased by 34.07% compared with the control (p<0.01). Compared with the control, the survival time of F1~F4 generations female and male fruit flies under the acute oxidative damage of paraquat were also prolonged (7.06 and 6.28 h (p<0.01), respectively). At the same time, the activities of SOD and CAT in Drosophila generations increased (p<0.01), and the content of MDA was reduced (p<0.01). It indicated that high dose of Codonopisis pilosula polysaccharides could increase the reproduction capability and extend the life span of F1 to F4 female and male Drosophila generations. After treated with the high dose of Codonopisis pilosula polysaccharides, the survival time in paraquat treatment was improved, the contents of MDA in the Drosophila generations was reduced and the activity of SOD and CAT or the antioxidant capacity increased rapidly, exhibiting a dosage effect., and the sex ratio of female and male Drosophila generations maintain 1:1.

陇南市科技指导性计划项目(2016-09)；甘肃省高等学校创新能力提升项目(2019A-190)