为了从高氨氮养殖废水中发掘高效脱氮菌株资源，本研究采用富集培养的方法，从猪粪水自然曝气池污水中分离得到1株有较高脱氮效率异养硝化菌ZF1-1；经形态学分析、16S rRNA基因序列比对和系统发育树构建，鉴定其为Bacillus siamensis ZF1-1。脱氮特性研究表明，在以硫酸铵为唯一氮源的人工废水培养基中，菌株ZF1-1在接种量1%、30 ℃、180 r/min培养72 h的条件下，对氨氮的转化率为77.55%，总氮脱除率为21.00%，且不积累中间产物硝酸盐和亚硝酸盐。菌株ZF1-1转化氨氮最适碳源是甘露醇，最适碳氮比为20。相同培养条件和培养时间下，初始氨氮浓度增加，则氨氮脱除率降低；初始氨氮浓度为100 mg/L时，氨氮转化效果最好，转化率达93.46%。Fe2+、Fe3+和Mg2+能显著提高菌株ZF1-1的氨氮脱除率，分别达到98.66%、93.48%、86.47%。将菌株ZF1-1应用于高氨氮浓度（1277.41 mg/L）的猪粪废水脱氮；结果显示，菌株ZF1-1处理效果较好，使猪粪废水氨氮和总氮浓度分别降低37.50%和22.22%。因此，菌株ZF1-1在畜禽养殖高氨氮污染废水脱氮领域具有良好的应用价值。

In order to excavate high-efficiency nitrogen-removing strains from high ammonia nitrogen breeding wastewater, a heterotrophic nitrifying strain ZF1-1 with high denitrification efficiency was isolated from the natural aeration tank sewage of pig manure-water using enrichment culture method. Based on morphological analysis, 16S rRNA gene sequence alignment and phylogenetic tree construction, strain ZF1-1 was identified as Bacillus siamensis. The denitrification characteristics of ZF1-1 showed that, in an artificial wastewater culture medium using ammonium sulfate as the only nitrogen source, the strain had a conversion rate of 77.55% to ammonia nitrogen, a total nitrogen removal rate of 21% and no nitrate and nitrite (intermediate products) accumulation when the inoculum amount was 1% at 30 ℃, 180 r/min for 72 h. The optimum carbon source for nitrifying ammonia nitrogen of strain ZF1-1 is mannitol, and the optimum carbon-nitrogen ratio is 20. Increasing initial concentration of ammonia nitrogen resulted in decreasing of ammonia nitrogen conversion rate under the same experimental conditions and incubation time. The highest conversion rate of ammonia nitrogen is 93.46% when the initial ammonia nitrogen concentration reaches 100 mg/L. Fe2+, Fe3+ and Mg2+ can significantly improve the conversion rate of ammonia nitrogen for of 98.66%, 93.48% and 86.47% respectively. Subsequently strain ZF1-1 was applied to remove nitrogen (1277.41 mg/L) from pig manure-water with high ammonia nitrogen concentration. The results reveal that strain ZF1-1 exhibit a satisfactory treatment effect, can reduce the ammonia nitrogen and total nitrogen concentrations to 37.50% and 22.22% respectively in the wastewater. Therefore, the strain ZF1-1 has good application potential for nitrogen removal in high ammonia nitrogen polluted wastewater from livestock and poultry breeding.

广东省重点领域研发计划项目（2019B020215001）；广东省科技计划项目（2019B030316009）；肇庆市科技计划项目（2019G010）；广州市科技计划项目珠江科技新星专题（201806010065，201906010022）；广东省自然科学基金项目（2018A030313476）