[关键词]
[摘要]
本研究探讨了杜仲叶多糖对糖尿病大鼠的降血糖作用。建立糖尿病模型大鼠,采用杜仲叶多糖干预。检测其清除DPPH、ABTS自由基能力,对α-葡萄糖苷酶抑制活性及空腹血糖(FBG)、尿素氮(BUN)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、Caspase-3、p38MAPK及TGF-β1蛋白水平。结果表明,杜仲叶多糖浓度为1 mg/mL时,其DPPH自由基清除率为65.15%、对ABTS自由基清除率49.68%。杜仲叶多糖高剂量组的Iα-glucosidase为90.47%,FBG和BUN分别为9.21 mmol/L、6.85 mmol/L,与其他各组差异显著(p<0.05)。杜仲叶多糖高剂量组的MDA、GSH-Px和SOD分别为7.27 μmol/L、54.27 U/mg和81.27 μg/mL,均明显好于其他实验组(p<0.05)。杜仲叶多糖高剂量组的Caspase-3、p38MAPK TGF-β1分别为0.70、0.69、0.71,显著低于阳性对照组(p<0.05)。研究结果表明,杜仲叶多糖可明显降低糖尿病大鼠FBG水平,具有较好的抗氧化作用,对糖尿病大鼠的胰岛细胞具有一定的保护作用。
[Key word]
[Abstract]
In this study, the hypoglycemic effect of the polysaccharide Eucommia ulmoides leaves in diabetic rats was investigated. Diabetic rat model was established and treated with the polysaccharide obtained from Eucommia ulmoides leaves. The scavenging capacities for DPPH and ABTS free radicals, the inhibitory activity against α-glucosidase, and the levels of fasting blood glucose (FBG), blood urea nitrogen (BUN), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), caspase-3, p38MAPK and TGF-β1 protein were measured. The results showed that the DPPH and ABTS radical scavenging rates were 65.15% and 49.68%, respectively, when the Eucommia ulmoides leaf polysaccharide concentration was 1 mg/mL. For the group treated with a high-dose of Eucommia ulmoides leaf polysaccharide, the Iα-glucosidase, FBG and BUN were 90.47%, 9.21 mmol/L and 6.85 mmol/L, respectively, which were significantly different from those of the other groups (p<0.05). The MDA, GSH-Px and SOD of the high-dose group were 7.27 μmol/L, 54.27 U/mg, and 81.27 g/mL, respectively, which were all better than the other experimental groups (p<0.05). The caspase-3, p38MAPK and TGF- β1 of the high-dose group were 0.50, 0.49, and 0.51, respectively, which were significantly lower than those of the positive control group (p<0.05). The research results show that the polysaccharide from Eumoides ulmoides leaves can significantly reduce the level of FBG in diabetic rats, exhibit a significant antioxidant effect, and provide a protective effect on the islet cells of diabetic rats.
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[基金项目]
云南省教育厅科学研究基金项目(2019J1120)