本研究探讨了贻贝、牡蛎复合物对抗生素诱导小鼠便秘的干预作用，采用混合抗生素溶液灌胃C57BL/6雌性小鼠，构建肠道菌群紊乱型小鼠便秘模型（ME），以0.02 mL/g·bw贻贝、牡蛎复合物灌胃ME组小鼠为干预组（IC）。结合16s RNA基因测序技术，7 d后，测定小肠推进率、排便量、饲料消耗量、肠道菌群结构等指标，评估小鼠便秘模型及其贻贝、牡蛎复合物的干预作用。对比空白组，ME组小肠推进率下降22.89%（p<0.05），排便量降低41.00%（p<0.05），日平均饲料消耗量显著下降2.68 g/d（p<0.05），粪便含水率显著下降19.40%（p<0.05），同时，ME组肠道菌群有益菌多样性减少，表现为Prevotella-9、Lachnospiraceae、Enterobacter、Verrucomicrobia等有益菌菌群丰度降低；经贻贝、牡蛎复合物干预后，对比ME组，IC组小鼠小肠推进率提升70.00%（p<0.05），排便量提升52.00%（p<0.05），粪便含水率显著增加18.10%（p<0.05），IC组肠道菌群多样性增加，表现为Proteobacteria、Fusobacteria、Akkermansia、Bacteroidetes、Verrucomicrobia丰度增加，同时IC组肠道菌群结构恢复至与空白组小鼠相似。综上，贻贝、牡蛎复合物可显著改善由抗生素导致的便秘小鼠的小肠推进率等小肠蠕动功能，增加小鼠排便，并提高粪便含水率，同时调节便秘小鼠的肠道菌群结构及组成，增加Akkermansia等肠道有益菌。

In this study, the intervention effect of mussel and oyster compound on antibiotic-induced constipation in mice was investigated. C57BL/6 female mice were administered via gavage with the mixed antibiotic solution, to establish a mouse constipation model with intestinal flora disorder (ME). The intervention group (IC) was intragastrically administered via gavage with mussel and oyster compound (0.02 mL/g·bw). Combined with the 16s RNA gene sequencing technology, the indices including the small intestinal propulsion rate, defecation volume, feed consumption, intestinal flora structure were measured after 7 days to evaluate the mouse constipation model, and the intervention effect of mussel and oyster compound. Compared with the blank group, the small intestine propulsion rate of the ME group decreased by 22.89% (p<0.05), the defecation amount decreased by 41.00% (p<0.05), the average daily feed consumption decreased by 2.68 g/d (p<0.05), and the water content of feces decreased by 19.40% (p<0.05). In the meantime, the diversity of beneficial bacteria in the intestinal flora decreased in ME group, which was manifested in the decrease in abundance of beneficial bacteria such as Prevotella-9, Lachnospiraceae, Enterobacter and Verrucomicrobia. After the intervention with mussel and oyster compound, the IC group had an increased (by 70.00%; p<0.05) small intestine propulsion rate, increased (by 52.00%; p<0.05) defecation volume, increased (by 18.10%; p<0.05) fecal water content, and increased diversity of intestinal flora (as increased abundance of Proteobacteria, Fusobacteria, Akkermansia, Bacteroidetes and Verrucomicrobia), compared with ME group. Also, the composition and structure of the intestinal flora in IC group were similar to those of the blank group. In summary, the mussel and oyster compound could significantly improve the small intestinal peristalsis function of mice with constipation caused by antibiotics, and increase the defecation amount and fecal water content, while regulating the structure and composition of intestinal flora of the mice with constipation and increasing the intestinal beneficial bacteria such as Akkermansia.

广东省现代农业产业技术体系共性关键技术创新团队建设专项资金项目（N0.2019KJ151）；广东省科技厅公益研究与能力建设专项资金项目（2014B020204005）