[关键词]
[摘要]
本研究以SPF级SD大鼠为实验动物,探讨刺葡萄酒对高脂饲料喂养大鼠的降血脂作用及可能机制。结果显示,刺葡萄酒能显著降低甘油三酯含量,低剂量刺葡萄酒能显著降低大鼠血清内总胆固醇至1.67 mmol/L(p<0.05);中剂量刺葡萄酒处理能显著降低密度脂蛋白胆固醇含量(p<0.01)并显著增加高密度脂蛋白胆固醇含量至0.97 mmol/L(p<0.05)。各剂量刺葡萄酒处理能显著增加大鼠血清内超氧化物歧化酶和谷丙转氨酶(p<0.01)的活性,显著降低血清丙二醛的含量(p<0.05)。Q-PCR结果显示,刺葡萄酒可促进高脂饮食大鼠肝脏卵磷脂胆固醇脂酰转移酶(LCAT)(1.47倍)、丙二酰辅酶A脱羧酶(MCD)(2.21倍)、肉碱棕榈酰转移酶(CPT-1)(1.31倍)的表达,抑制酯酰辅酶A:胆固醇酯酰转移酶(ACAT1)(1.95倍)、DGAT2(1.43倍)、线粒体解偶联蛋白2(UCP2)(1.98倍)等负调控基因的上调表达。本研究的结果证明适量刺葡萄酒可降低高脂饮食大鼠体内血脂水平,促进肝脏脂质代谢,抵抗高脂诱导的脂质过氧化。并且,该作用的发挥可能与刺葡萄酒调节肝脏脂质代谢关键基因表达与脂质代谢的相关酶活性有关。
[Key word]
[Abstract]
In this study, SPF grade SD rats were used as experimental animals to study the hypolipidemic effects and possible mechanism of red wine made from V. didii. Fo?x. The results showed that the content of triglyceride in the serum of rats was significantly reduced by V. didii. Fo?x Wine, and the content of total cholesterol in the serum of rats was significantly reduced to 1.67 mmol/L under the low dose (p<0.05); the content of LDL-C was significantly reduced under medium dose of V. didii. Fo?x Wine (p<0.01), and the content of HDL-C was significantly increased to 0.97 mmol/L under the medium dose (p<0.05). Treatments with V. didii. Fo?x wine significantly increased the activities of SOD and GSH-Px (p<0.01), but reduced the content of MDA in the serum (p<0.05). The results of Q-PCR showed that red wine promoted the expression of LCAT (1.47 times), MCD (2.21 times), and CPT-1 (1.31 times) genes in high-fat rats, and inhibited the up-regulation of negatively regulated genes such as ACAT1 (1.95 times), DGAT2 (1.43 times), and UCP2 (1.98 times). The results of this study demonstrate that proper amount of V. didii. Fo?x Wine can reduce blood lipid levels in rats on a high-fat diet, promote liver lipid metabolism, and resist to high-fat induced lipid peroxidation. Moreover, the hypolipidemic effects of V. didii. Fo?x Wine may be related to the regulation of key gene expression of liver lipid metabolism and related enzyme activity.
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[基金项目]
国家现代农业产业技术体系(CARS-29-ZP-9);湖南省教育厅科学研究项目(17C0768)