以红地球葡萄为研究试材，采用（0、50、100和200 μmol/L）茉莉酸甲酯（Methyl Jasmonate，MeJA）在膨大期、转色期和采收前2 d（预收期）喷施处理红提葡萄果实，在0±1 ℃条件下贮藏。结果表明，在离体条件下，MeJA对灰葡萄孢菌的菌体生长量和菌落直径无显著差异作用（p<0.05），较高浓度对菌体生长具有一定的抑制作用。在贮藏期间，MeJA可以显著抑制葡萄果实采后腐烂的发生、延缓果实硬度的下降和提高果实可溶性固形物的含量，维持可滴定酸含量。100 μmol/L MeJA处理组优于其他组，在贮藏50 d时，CK组发病率为86.09%，是MeJA处理组的1.53倍，MeJA处理组的可溶性固形物含量为17.3%，可滴定酸含量为0.675%。40 d时MeJA处理组的硬度为7.5 N，是CK组的1.59倍，CHI活性为370.12 U/g，20 d时MeJA处理组GLU活性为105.86 U/g，是CK组的1.11倍。10 d时MeJA处理组PAL和POD活性为2.43 U/g和0.506 U/g，是CK组的1.18和1.58倍。MeJA采前喷施能有效诱导果实PAL、POD、CHI和GLU酶活性的上升，表明MeJA采前喷施处理能够通过诱导提高葡萄实酶活性来提高果实采后抗病性，提高葡萄贮藏效果，减少果实采后腐烂的发生。

Red Globe grapes were used as the research materials. Methyl jasmonate (MeJA; 0, 50, 100, and 200 μmol/L) was sprayed during the expansion period, the color change period, and 2 days before harvest (pre-harvest period). The treated fruits were stored at 0±1 ℃. The results showed that under the in vitro conditions, MeJA had insignificant effect on the growth and colony diameter of Botrytis cinerea (p< 0.05), although higher concentrations of MeJA had a certain inhibitory effect on its growth. During storage, MeJA can significantly inhibit the occurrence of post-harvest fruit decay, delay the decline of fruit hardness, increase the content of fruit soluble solids, and maintain the titratable acid content of fruits. The MeJA treatment at 100 μmol/L worked better than those at other concentrations. At Day 50 of storage, the incidence rate of the CK group was 86.09%, which was 1.53 times that of the MeJA treatment group (which had a soluble solids content of 17.3% and a titratable acid content of 0.675%). At Day 40, the fruit hardness for the MeJA treated group was 7.5 N (which was 1.59 times that for the CK group), and the CHI activity of the MeJA treated group was 370.12 U/g. At Day 20 the GLU activity of the MeJA-treated group was 105.86 U/g (which was 1.11 times that of the CK group). At Day 10, the PAL and POD activities for the MeJA treatment group were 2.43 U/g and 0.506 U/g, which were 1.18 and 1.58 times, respectively, those of the CK group. Preharvest MeJA spraying can effectively induce the increase of PAL, POD, CHI and GLU activities of the fruits, indicating that this treatment can increase the resistance to post-harvest diseases, improve grape storage outcome and reduces post-harvest decay through inducing and increasing the enzymatic activities in grapes.

国家重点研发计划项目（2018YFD0401302）；国家自然科学基金项目（31860460）；新疆农业科学院青年基金项目（xjnkq-2016020）