本研究拟探讨致病小龙虾头与尾匀浆在诱导SD大鼠发生食源性哈夫病相关重要生化指标变化中的作用。将深圳市一起食源性哈夫病病人食用的同一来源小龙虾头（肝胰腺）和尾（虾肉）分开并高温处理后对SD大鼠进行灌胃，不同时间点取血液后分离血清进行肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）及乳酸脱氢酶（LDH）等多种生化指标检测；分析小龙虾头与尾匀浆在不同时间点诱导SD大鼠哈夫病诊断指标出现变化的情况。结果显示，肝胰腺灌胃24 h后肝胰腺组大鼠血清中CK（613±220.6）、CK-MB（509±196.1）和LDH（1044±672.8）出现超过对照组2倍标准差的异常值，P分别为0.523、0.417和p=0.237。灌胃48 h后，虾肉组大鼠血清中CK（78±12.5）、CK-MB（78±12.5）和LDH（954±694.2）出现超过对照组2倍标准差的异常值，p分别为0.11、0.11和0.87。通过灌胃处理后不同时间点检测相关重要生化指标，初步鉴定出哈夫病诊断相关重要指标出现异常的大鼠，可为食用小龙虾导致哈夫病的快速诊断及其致病机理提供科学线索。

Head and tail homogenates of crayfish were administered to Sprague-Dawley rats in order to investigate the changes induced in several key biochemical indicators associated with seafood-induced Haff disease; namely, creatine kinase (CK), creatine kinase MB (CK-MB), and lactate dehydrogenase (LDH). The crayfish used in this study was obtained from the same source that was responsible for a case of Haff disease in Shenzhen, China. The head (hepatopancreas) and tail (crayfish meat) of the crayfish were separately subjected to high-temperature sterilization before their administration to the rats by oral gavage. Blood samples were then collected from the rats at different time points following the oral treatment, and the serum was separated for measurement of the CK, CK-MB, and LDH levels. The rats administered the hepatopancreas homogenate had serum CK (613±220.6), CK-MB (509±196.1), and LDH (1044±672.8) levels that were higher than those of the control group by more than two standard deviations (P = 0.523, 0.417, and 0.237, respectively) at 24 h after oral treatment. The rats administered the crayfish meat homogenate had serum CK (78±12.5), CK-MB (78±12.5), and LDH (954±694.2) levels that were higher than those of the control group by more than two standard deviations (P = 0.11, 0.11, and 0.87, respectively) at 48 h after oral treatment. Such measurement of relevant biochemical indicators at different time points after oral gavage administration of the “pathogen” allowed for the preliminary identification of rats with Haff-like disease on the basis of abnormal indicator values. These results provide a scientific basis for the rapid diagnosis of Haff disease induced by crayfish consumption and for the elucidation of the disease pathogenesis.

深圳市医疗卫生三名工程项目(SZSM201611090)；广东省自然科学基金项目（2017A030310617）