[关键词]
[摘要]
为了研究乌鳢胶原肽在6种不同离子强度诱导下聚集的结构与特性的变化规律,对聚集体变化过程中的内源荧光、浊度、微观结构、粒径、黏度及其二级结构进行测定。结果表明,在0~500 mM的NaCl范围内,离子强度具有促进胶原蛋白肽自组装聚集的效果,且聚集速率随离子强度的增大而增加。在0~150 mM范围内,胶原蛋白肽聚集的三维网络紧密性增加,聚集体平均粒径增大74.42%,最大剪切黏度增加487.67%。当离子强度在150~500 mM范围时,胶原蛋白肽三维网络紧密性降低,聚集体平均粒径下降,最大剪切黏度降低21.39%。胶原蛋白肽聚集体的二级结构并没有随离子强度变化而发生明显变化。因此,适度的增大样品离子强度可促进胶原肽的自组装聚集,改善其凝胶的三维网络结构和理化特性,为胶原蛋白肽进一步应用于制备药物载体的研究提供参考。
[Key word]
[Abstract]
In order to determine the effect of Channa argus collagen peptide (CP) of self-assemble aggregation on the change of structure and characteristics at 6 different ionic strengths, the intrinsic fluorescence, turbidity, microstructure, diameter, viscosity and secondary structure of the aggregate were measured. The results showed that the self-assembly and aggregation of CP can be promoted by ionic strength at the range of 0~500 mM, and the aggregation rate was increased with the increasing ionic strength. The three-dimensional network compactness of CP self-assembly increased, and the average particle size of the aggregate increased by 74.42%, and the maximum shear viscosity increased at the range of 0~150 mM. The three-dimensional network compactness of CP decreased, the average particle size of the aggregate decreased, and the maximum shear viscosity decreased by 21.39% at the range of 150~500 mM of ionic strength. However, the secondary structure of CP had not changed significantly. In conclusion, the self-assembly process of CP can be promoted and its structure and physicochemical properties can be effectively improved when the ionic strength of samples was moderately increased. The results of this study can provide a reference for the further application of CP in the preparation of drug carriers.
[中图分类号]
[基金项目]
国家自然科学基金面上项目(31972021),广东省自然科学基金重点项目(2018B0303110006),广东省国际合作项目(2018A050506048),广东省重点研发项目(2019B020212003),广东省促进经济高质量发展专项(MEPP[2019]A38),广东省现代农业产业技术体系创新团队建设专项(2019KJ117),广东省农科院人才项目(R2018PY-JX002),广东省教育厅科技项目(2017GKSCX057)