本研究探讨了广东地区副猪嗜血杆菌的亲缘关系、药物敏感性及耐药基因的携带现况，对分离自广东不同区域的53株副猪嗜血杆菌进行了16S rRNA进化分析，使用K-B琼脂扩散法针对21种抗菌药物进行敏感性检测，并通过全基因组测序分析其耐药基因携带情况。结果表明，菌株对苯唑西林、复方新诺明、链霉素和四环素耐药率较高，且多重耐药菌株占比较高（84.91%）。通过与耐药基因数据库对比共检测到21种耐药基因，介导9大类抗生素的耐药，其中春日霉素耐药基因ksgA和杆菌肽耐药基因bacA首次在副猪嗜血杆菌被发现。氨基糖苷类和林可霉素类耐药基因携带情况与耐药表型之间符合率可达100%。16S rRNA分析结果显示，不同区域来源的副猪嗜血杆菌亲缘关系较近，且耐药基因携带情况与16S rRNA进化关系以及分离时间存在一定的关联性。本研究结果可对广东地区养猪业副猪嗜血杆菌疾病的防御和治疗起到指导作用。

In this study, the genetic relationship, drug sensitivity, and drug resistance genes carried by Glaesserella parasuis in Guangdong province were investigated. The evolution of 16S rRNA of the 53 Glaesserella parasuis strains isolated from different regions in Guangdong was analysed , and their susceptibility to 21 antibacterial drugs was analyzed using the K-B disc diffusion method and the carried drug resistance genes were analyzed using the whole genome sequencing (WGS). Results showed that the strains had high drug resistance rates to oxacillin, trimethoprim-sulfamet, streptomycin and tetracycline, and the multi-drug resistant strains accounted for a relatively high rate (84.91%). A comparison with the drug resistance gene database revealed 21 drug resistance genes that mediate resistance to 9 major classes of antibiotics, among which kasugamycin-resistant gene, ksgA, and bacitracin-resistant gene, bacA, were detected for the first time. The coincidence rate between the aminoglycoside and lincomycin resistance genes and the resistance phenotypes reached 100%. The 16S rRNA gene analysis a close genetic relationship between the Glaesserella parasuis strains from different regions, and a correlation between to the carried drug-resistant genes and the 16S rRNA evolution and isolation time. The results of this study can be used to guide the prevention and treatment of Glaesserella parasuis diseases in Guangdong.

国家自然科学基金资助项目（31571934；31772776）