本研究建立一种测定番茄红素原料及保健食品中番茄红素、β-胡萝卜素含量的通用方法。一般类型样品用含2,6-二叔丁基对甲酚的二氯甲烷溶液（BHT溶液）直接溶解，微囊化样品先用含2,6-二叔丁基对甲酚的N,N-二甲基甲酰胺超声提取后再用BHT溶液溶解稀释，后均采用高效液相色谱检测分析，在40 min内完成目标化合物的分离检测。在实验条件下，番茄红素和β-胡萝卜素分离度和线性关系良好，检出限分别为0.08 μg/mL和0.09 μg/mL。一般类型样品重复性实验番茄红素和β-胡萝卜素的相对标准偏差（RSD）分别为3.20%和3.01%；微囊化样品重复性实验番茄红素的RSD为2.37%，β-胡萝卜素未检出。两类样品番茄红素的加标回收率在92.65%~107.49%之间，β-胡萝卜素的加标回收率在94.73%~105.12%之间。该方法稳定、准确、适应性广，基本涵盖所有类型番茄红素原料及保健食品中番茄红素和β-胡萝卜素含量的测定。

In this study, a universal method for determining the contents of lycopene and β-carotene in lycopene-containing raw materials and functional foods was established. In general, ordinary samples were dissolved directly in a dichloromethane solution containing 2, 6-di-tert-butyl-4-methylphenol (BHT solution), while the microencapsulated samples were firstly subjected to ultrasonic-assisted extraction with N,N-dimethyl formamide containing 2,6-di-tert-butyl-4-methylphenol, and then dissolved and diluted with BHT solution. High performance liquid chromatography was used for identification and analysis, and the separation and quantification of the target compounds were completed in 40 min. Under the experimental conditions, the degree of separation and the linear relationship of lycopene and β-carotene were satisfactory, with corresponding limits of detection being 0.08 μg/mL and 0.09 μg/mL, respectively. The relative standard deviations (RSDs) of lycopene and β-carotene for the ordinary samples were 3.20% and 3.01%, respectively. The RSD of lycopene in microencapsulated samples was 2.37%, and no β-carotene was detected. The recovery rate of lycopene in the two types of samples ranged from 92.65% to 107.49%, and that of β-carotene ranged from 94.73% to 105.12%. This method is stable, accurate, and adaptable, and can be essentially applied to the determination of almost all kinds of lycopene-containing raw materials and functional foods.

保健食品原料目录研究专项课题项目（GXTC-1770075）