张彩芹,张勋,李洁,李娜,肖香,欧阳琴,王云,周兴华.胶体金试纸条同时检测鲤鱼中7种苯并咪唑类药物的残留[J].,2020,36(6):291-296.
胶体金试纸条同时检测鲤鱼中7种苯并咪唑类药物的残留
Simultaneous Detection of 7 Benzimidazoles Residues in Carp by Colloidal Gold Immunochromatographic Strip
投稿时间:2019-12-22  
DOI:10.13982/j.mfst.1673-9078.2020.6.1263
中文关键词:  苯并咪唑  单克隆抗体  胶体金试纸条  鲤鱼
英文关键词:benzimidazoles  monoclonal antibody  colloidal gold immunochromatographic strip  carp
作者简介:张彩芹(1993-),女,硕士研究生,研究方向:食品安全检测 通讯作者:周兴华(1982-),女,博士,副教授,研究方向:食品安全检测
基金项目:国家重点研发计划项目(2017YFC1600801)
作者单位
张彩芹 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
张勋 (2.江苏美正生物科技有限公司,江苏无锡 214000) 
李洁 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
李娜 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
肖香 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
欧阳琴 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
王云 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
周兴华 (1.江苏大学食品与生物工程学院,江苏镇江 212013) 
AuthorInstitution
ZHANG Cai-qin (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
ZHANG Xun (2.Jiangsu MeiZheng Biological Technology Co. Ltd., Wuxi 214000, China) 
LI Jie (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
LI Na (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
XIAO Xiang (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
OUYANG Qin (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
WANG Yun (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
ZHOU Xing-hua (1.School of Food and Biological Engineering, Jiangsu University, Zhengjiang 212013, China) 
摘要点击次数: 11
全文下载次数: 16
中文摘要:
      为检测鲤鱼中苯并咪唑类药物残留,降低其对人体造成的危害,本文基于单克隆抗体之上建立胶体金试纸条检测方法。首先以2-甲氧基羰基氨基-3H-苯并咪唑-5-羧酸为半抗原,用活化酯法偶联蛋白质制备免疫原,免疫小鼠,进行细胞融合制备单克隆抗体,而后用合成的胶体金标记单克隆抗体制备胶体金试纸条。通过间接竞争ELSIA法(ic-ELISA)测得阿苯达唑、甲苯咪唑、阿苯达唑亚砜、阿苯达唑砜、芬苯达唑、氟苯咪唑、奥芬达唑的半抑制浓度(IC50)分别为0.44、0.16、3.47、5.62、0.62、0.10、5.77 ng/mL。胶体金试纸条在鲤鱼中的阿苯达唑、甲苯咪唑、阿苯达唑亚砜、阿苯达唑砜、芬苯达唑、氟苯咪唑、奥芬达唑的检测限分别为4、2、50、100、10、1、100 ng/g,检测时间为15 min。因此,该方法灵敏度高、成本低、速度快且无需仪器辅助,适宜现场大量鲤鱼样本的快速检测。
英文摘要:
      In order to reduce its harm to human, a colloidal gold immunochromatographic strip was established based on monoclonal antibody (mAb) to detect the residue of benzimidazoles in carp. Firstly, the immunogen was prepared using 2-(methoxycarbonylamino)- 3H-benzimidazole-5-carboxylic acid as hapten and coupling protein by activated ester method, mice were immunized and mAb was prepared by cell fusion. Then A colloidal gold immunochromatographic strip was prepared by mAb labelled with synthetic colloidal gold. The 50% inhibitory concentrations (IC50) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole flubendazole, oxfendazole were 0.44, 0.16, 3.47, 5.62, 0.62, 0.10, 5.77 ng/mL by ic-ELISA, respectively. The limit of detection (LOD) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole, flubendazole, oxfendazole were 4, 2, 50, 100, 10, 1, 100 ng/g in carp samples, respectively. Therefore, the developed method was highly sensitive, rapid, low-cost, free-instrument and applicable for on-site detection of a large numbers of carp samples.
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