岳苑,周梦诗,徐娟,李睿,何利华,赵飞,张建中,龚杰.基于高分辨率溶解曲线分析鉴别食品中的3种李斯特氏菌[J].,2020,36(6):285-290.
基于高分辨率溶解曲线分析鉴别食品中的3种李斯特氏菌
Identification of Three Listeria Species in Foods based on High Resolution Melting Curve Analysis
投稿时间:2019-12-02  
DOI:10.13982/j.mfst.1673-9078.2020.6.1176
中文关键词:  高分辨率熔解曲线  单核细胞增生李斯特氏菌  伊氏李斯特氏菌  英诺克李斯特氏菌  食品
英文关键词:high-resolution melting curve  Listeria monocytogenes  Listeria ivanovii  Listeria innocua  foods
作者简介:岳苑(1982-),女,博士研究生,高级工程师,研究方向:病原微生物 通讯作者:龚杰(1985-),男,博士,助理研究员,研究方向:分子诊断学
基金项目:“十三五”国家科技重大专项课题(2018ZX10712-001);传染病预防控制国家重点实验室重点项目(2014SKLID102)
作者单位
岳苑 (1.宁夏回族自治区食品检测研究院,宁夏银川 750001)(2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206) 
周梦诗 (3.西安博睿康宁生物医学中心有限公司,陕西西安 710000) 
徐娟 (2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206)(4.武汉科技大学医学院公共卫生学院,湖北武汉 430000) 
李睿 (3.西安博睿康宁生物医学中心有限公司,陕西西安 710000) 
何利华 (2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206) 
赵飞 (2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206) 
张建中 (2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206) 
龚杰 (2.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206) 
AuthorInstitution
YUE Yuan (1.Ningxia hui autonomous region food testing and research institute, Yinchuan 750001, China) (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) 
ZHOU Meng-shi (3.Xi'an biorealcoming biomedical center co. LTD, Xi 'an 710000, China) 
XU Juan (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) (4.School of Public Health, Wuhan University of Science and Technology, Wuhan 430000, China) 
LI Rui (3.Xi'an biorealcoming biomedical center co. LTD, Xi 'an 710000, China) 
HE Li-hua (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) 
ZHAO Fei (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) 
ZHANG Jian-zhong (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) 
GONG Jie (2.Institute of infectious disease control and prevention, Chinese center for disease control and prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China) 
摘要点击次数: 16
全文下载次数: 19
中文摘要:
      本研究利用高分辨率熔解曲线的方法,以iap基因为靶标新设计一对引物同时鉴别单增李斯特氏菌、伊氏李斯特氏菌和英诺克李斯特氏菌,其余14种常见食源性病原微生物扩增为阴性结果,单增李斯特氏菌和伊氏李斯特氏菌的检出限为10个拷贝,英诺克李斯特氏菌的检测限为50个拷贝。本研究对78份样品进行HRM-real time PCR法、GB4789.30-2016和SN/T 1870-2016(荧光PCR法)的检测,并对3种方法的检测结果进行统计学分析。结果显示:HRM法和国标方法、HRM法和荧光PCR法的检测结果之间存在统计学差异,国标方法和荧光PCR法检测结果之间无统计学差异。本研究建立的基于HRM-real time PCR法检测3种李斯特氏菌的方法快速高效、特异性好、成本低,适用于食品中李斯特氏菌的日常检测和监管。
英文摘要:
      In this study, a new pair of primers was designed to identify Listeria monocytogenes, Listeria evanescens and Listeria innominata at the same time with iap gene as the target by using high-resolution fusion curve method. The amplification of the other 14 common foodborne pathogens was negative. The detection limit of Listeria monocytogenes and Listeria evanescens was 10 copies, and that of Listeria innominata was 50 copies. In this study, 78 samples were tested by HRM-real time PCR method, GB4789.30-2016 and SN/T 1870-2016 (real-time PCR), and the results of three methods were analyzed statistically. The results showed that there were statistical differences between the detection results of HRM method and national standard method, HRM method and fluorescent PCR method, and there was no statistical difference between the results of national standard method and fluorescent PCR method. The method based on HRM-real time PCR for the detection of three Listeria species in this study is fast, efficient, specific, and low cost. It is suitable for the daily detection and supervision of Listeria species in food.
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