以大豆卵磷脂和胆固醇作为壁材，采用薄膜分散-高压均质法制备佛手精油脂质体。以脂质体粒径、Zeta电位和包封率为指标筛选工艺参数和配方参数，确定脂质体最佳制备工艺，并对脂质体的形貌、稳定性进行考察，同时研究其抗氧化活性。结果表明，佛手精油脂质体的最佳制备工艺参数为：均质压力120 MPa、均质次数5次、磷脂/胆固醇比例5:1、精油添加量3.5 mg/mL。在此条件下制备的脂质体粒径为183.53 nm，PDI多分散指数为0.147，Zeta电位值为38.75 mV，包封率为47.23%，载药量为1.67 mg/mL。所制备的脂质体具有良好的离心稳定性、贮藏稳定性4 ℃贮藏30 d，精油保留率为85.03%，对DPPH、ABTS、羟基自由基的清除率分别为82.58%、78.02%、70.89%。结论：在均质压力120 MPa、均质次数5次、磷脂/胆固醇比例5:1、精油添加量3.5 mg/mL的条件下，制备的脂质体粒径适中、稳定性良好，具有良好的抗氧化活性。

Using soybean lecithin and cholesterol as wall materials, bergamot essential oil liposomes were prepared by membrane dispersion and high pressure homogenization. The parameters of preparation and formulation were selected according to the particle size, zeta potential and entrapment efficiency of liposome, and the optimal preparation process was determined. The morphology and stability of liposome were investigated, and its antioxidant activity was also studied. The optimal process parameters were obtained as follows：Homogenization pressure 120 MPa, homogenization times 5 times, phospholipid/cholesterol ratio 5:1, essential oil addition amount 3.5 mg/mL. The particle size of the liposome prepared under these conditions was 183.53 nm, the PDI polydispersity index was 0.147, the zeta potential value was 38.75 mV, encapsulation efficiency was 47.23%, and drug loading was 1.67 mg/mL. The liposomes had good centrifugation stability and storage stability for 30 days at 4 ℃, the retention rate of essential oil was 85.03%, and the scavenging rates of DPPH, ABTS and hydroxyl radicals were 82.58%, 78.02% and 70.89%, respectively. Conclusion: Under the conditions of homogenization pressure of 120 MPa, homogenization times of 5, ratio of phospholipid to cholesterol of 5:1, and the amount of essential oil of 3.5 mg/mL, the liposome prepared has moderate particle size, good stability and good antioxidant activity.

广东省“扬帆计划”引进创新创业团队项目（2016YT03S056）