韩佳悯,罗剑飞,林炜铁.酿酒废水硫氧化细菌的分离鉴定及其硫氧化特性[J].,2020,36(6):155-160.
酿酒废水硫氧化细菌的分离鉴定及其硫氧化特性
Isolation and Identification of Sulfur-oxidizing Bacterium from Brewery Wastewater and Its Sulfur-oxidizing Characteristics
投稿时间:2019-07-16  
DOI:10.13982/j.mfst.1673-9078.2020.6.0669
中文关键词:  化能自养型细菌  硫氧化作用  固氮作用
英文关键词:chemoautotrophic bacterium  sulfur oxidation  nitrogen fixation
作者简介:韩佳悯(1994-),女,硕士,研究方向:环境微生物 通讯作者:林炜铁(1964-),男,博士,教授,研究方向:微生物生态学、发酵工程等
基金项目:
作者单位
韩佳悯 (华南理工大学生物科学与工程学院,广东广州 510006) 
罗剑飞 (华南理工大学生物科学与工程学院,广东广州 510006) 
林炜铁 (华南理工大学生物科学与工程学院,广东广州 510006) 
AuthorInstitution
HAN Jia-min (School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China) 
LUO Jian-fei (School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China) 
LIN Wei-tie (School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China) 
摘要点击次数: 15
全文下载次数: 14
中文摘要:
      本研究从酿酒废水中分离了一株化能自养硫氧化细菌LS2,通过16S rRNA基因和硫氧化功能酶基因soxB分析表明该菌株与Halothiobacillus亲缘关系最近,但Halothiobacillus sp. LS2的基因组中存在完整的固氮酶基因簇nif,其关键编码基因nifH与Acidithiobacillus属中A. ferrooxidans和A. ferrivorans相似性最高,均高于80%。通过细胞水平的比较研究,菌株LS2在有氮和无氮条件下均可生长,在1 mM NH4+的条件下生长量最高可达1.98×107 /mL,而无氮条件下生长量较有氮条件下少,最高可达1.37×107 /mL;基于基因表达分析,菌株LS2的nifH表达量因氮源存在而下调,硫代硫酸盐浓度增加而上调,其中在5 mM S2O32-浓度下其表达量上调了约33倍。说明该菌的固氮作用与硫氧化反应息息相关。本研究获得了一株具有固氮能力的化能自养硫氧化细菌,所获得的结果可为含硫化物废水的治理提供一种新思路。
英文摘要:
      In this study, a chemoautotrophic sulfur-oxidizing bacterial strain LS2 was isolated from brewery wastewater. The analyses of 16S rRNA gene and sulfur-oxidizing enzyme gene soxB revealed that this strain was most closely related to genus Halothiobacillus, although the genome of Halothiobacillus sp. LS2 had a complete gene cluster encoding nitrogen-fixing enzyme Nif; Its key gene nifH had the highest similarity to A. ferrooxidans and A. ferrivorans in the genus Acidithiobacillus (both of which were higher than 80%). A comparative study at the cellular level indicated that the strain LS2 was able to grow under both nitrogen and nitrogen-free conditions. The maximum growth of LS2 under the condition of 1 mM NH4+ could reach 1.98×107/mL, whilst the growth in the absence of nitrogen (up to 1.37×107/mL) was less than that in the presence of nitrogen. The gene expression analysis revealed that nifH expression of strain LS2 was down-regulated due to the presence of a nitrogen source but up-regulated with the increase of thiosulfate concentration. The expression of nifH was up-regulated by 33 times at a S2O32-concentration of 5 mM, indicating that nitrogen fixation was closely related to sulfur oxidation. In this study, a chemoautotrophic sulfur-oxidizing bacterium with nitrogen-fixing ability was obtained, and the obtained results can provide a new idea for the treatment of sulfide-containing wastewater.
查看全文  查看/发表评论  下载PDF阅读器
关闭